Recombinant
RabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148]
    See all HLA Class II DRB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
    Database link: P01911

  • Positive control

    • Human tonsil tissue; Human spleen, tonsil, lymph node and Ramos cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6148
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody.
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : Human lymph node lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 29 kDa

    • Immunohistochemical analysis of HLA Class II DRB1 in formalin fixed, paraffin embedded Human tonsil tissue stained with ab133578 at a 1/100 dilution.
    • ab133578 showing positive staining in Diffuse large B-cell lymphoma tissue.

    • ab133578 showing positive staining in Normal colon tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kanangat S  et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . Read more (PubMed: 29656111) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 7.5% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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