Product nameAnti-HLA Class II DRB1 antibody [EPR6148]
See all HLA Class II DRB1 primary antibodies
DescriptionRabbit monoclonal [EPR6148] to HLA Class II DRB1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
Database link: P01911
- Human tonsil tissue; Human spleen, tonsil, lymph node and Ramos cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.80 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody.|
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 29 kDa.|
- Information by UniProt
- 2B1F_HUMAN antibody
- DR1 antibody
- DR16 antibody
All lanes : Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human lymph node lysate
Lane 3 : Ramos cell lysate
Lane 4 : Human tonsil lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 29 kDa
Immunohistochemical analysis of HLA Class II DRB1 in formalin fixed, paraffin embedded Human tonsil tissue stained with ab133578 at a 1/100 dilution.
ab133578 showing positive staining in Diffuse large B-cell lymphoma tissue.
ab133578 showing positive staining in Normal colon tissue.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD