Recombinant
RabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6148] to HLA Class II DRB1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148]
    See all HLA Class II DRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
    Database link: P01911

  • Positive control

    • WB: Human spleen, and Ramos lysates. IHC-P: human tonsil tissue. ICC/IF: Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6148

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use a HRP/AP polymerized secondary antibody.

See IHC antigen retrieval protocols

For unprified use at 1/100 - 1/250
ICC/IF 1/50.

For unprified use at 1/100 - 1/250
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Western blot - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      All lanes : Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578) at 1/1000 dilution (Purified)

      Lane 1 : Human spleen lysates
      Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 29 kDa
      Observed band size: 29 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

      Immunohistochemical analysis of HLA Class II DRB1 in formalin fixed, paraffin embedded Human tonsil tissue stained with ab133578 at a 1/100 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

      ab133578 showing positive staining in Diffuse large B-cell lymphoma tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

      ab133578 showing positive staining in Normal colon tissue.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Other - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Other - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    Publishing research using ab133578? Please let us know so that we can cite the reference in this datasheet.

    ab133578 has been referenced in 2 publications.

    • Bertram KM  et al. Identification of HIV transmitting CD11c+ human epidermal dendritic cells. Nat Commun 10:2759 (2019). PubMed: 31227717
    • Kanangat S  et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . PubMed: 29656111

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class II DRB1 antibody [EPR6148]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 7.5% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (Spleen)
    Specification
    Spleen
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 28 2014

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