For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    hla-class-ii-drb1-antibody-epr6148-ab133578.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity MHC Class II
Share by email
RecombinantRabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

  • Datasheet
Reviews (1) Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Western blot - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • OI-RD Scanning - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
  • Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6148] to HLA Class II DRB1
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
Product image
Recombinant Human HLA Class II DRB1 protein (denatured) (ab177593)
Primary
Product image
Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)

View more associated products

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148]
    See all HLA Class II DRB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
    Database link: P01911

  • Positive control

    • WB: Human spleen, and Ramos lysates. IHC-P: human tonsil tissue. ICC/IF: Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6148
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-HLA Class II DRB1 antibody [EPR6148] (ab207066)
    • Alexa Fluor® 488 Anti-HLA Class II DRB1 antibody [EPR6148] (ab207376)
    • Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human HLA Class II DRB1 protein (denatured) (ab177593)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use a HRP/AP polymerized secondary antibody.

See IHC antigen retrieval protocols

For unprified use at 1/100 - 1/250

ICC/IF
1/50.

For unprified use at 1/100 - 1/250

WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use a HRP/AP polymerized secondary antibody.

See IHC antigen retrieval protocols

For unprified use at 1/100 - 1/250

ICC/IF
1/50.

For unprified use at 1/100 - 1/250

WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Target information above from: UniProt accession P01911 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3123 Human
    • Omim: 142857 Human
    • SwissProt: P01911 Human
    • SwissProt: P01912 Human
    • SwissProt: P04229 Human
    • SwissProt: P20039 Human
    • SwissProt: Q29974 Human
    • SwissProt: Q30134 Human
    • SwissProt: Q30167 Human
    • SwissProt: Q5Y7A7 Human
    • SwissProt: Q95IE3 Human
    • SwissProt: Q9GIY3 Human
    • Unigene: 534322 Human
    • Unigene: 696211 Human
    • Unigene: 736560 Human
    see all
  • Alternative names

    • 2B1F_HUMAN antibody
    • DR1 antibody
    • DR16 antibody
    • DR5 antibody
    • DR8 antibody
    • DRB1 antibody
    • DRw10 antibody
    • DW2.2/DR2.2 antibody
    • FLJ75017 antibody
    • FLJ76359 antibody
    • HLA class II antigen beta chain antibody
    • HLA class II histocompatibility antigen, DR-1 beta chain antibody
    • HLA class II histocompatibility antigen, DRB1-15 beta chain antibody
    • HLA-DR-beta 1 antibody
    • HLA-DR1B antibody
    • HLA-DRB antibody
    • HLA-DRB1 antibody
    • human leucocyte antigen DRB1 antibody
    • Leucocyte antigen DR beta 1 chain antibody
    • leucocyte antigen DRB1 antibody
    • lymphocyte antigen DRB1 antibody
    • major histocompatibility complex, class II, DR beta 1 antibody
    • MHC class II antigen antibody
    • MHC class II antigen DRB1*15 antibody
    • MHC class II antigen HLA-DR13 antibody
    • MHC class II HLA-DR beta 1 chain antibody
    • MHC class II HLA-DR-beta cell surface glycoprotein antibody
    • MHC class II HLA-DRw10-beta antibody
    • SS1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Western blot - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    All lanes : Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578) at 1/1000 dilution (Purified)

    Lane 1 : Human spleen lysates
    Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

    Immunohistochemical analysis of HLA Class II DRB1 in formalin fixed, paraffin embedded Human tonsil tissue stained with ab133578 at a 1/100 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

    ab133578 showing positive staining in Diffuse large B-cell lymphoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

    ab133578 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    OI-RD Scanning - Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
    Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (3)

Publishing research using ab133578? Please let us know so that we can cite the reference in this datasheet.

ab133578 has been referenced in 3 publications.

  • Zhao X  et al. HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis. Reprod Biol Endocrinol 18:107 (2020). PubMed: 33160385
  • Bertram KM  et al. Identification of HIV transmitting CD11c+ human epidermal dendritic cells. Nat Commun 10:2759 (2019). PubMed: 31227717
  • Kanangat S  et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . PubMed: 29656111

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA Class II DRB1 antibody [EPR6148]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 7.5% · Temperature: 21°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Permeabilization
No
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.