Recombinant
RabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

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Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free
    See all HLA Class II DRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
    Database link: P01911

  • Positive control

    • IHC-P: human tonsil tissue. ICC/IF: Raji cells.

  • General notes

    ab248561 is the carrier-free version of ab133578 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248561 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6148

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab248561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)

    References

    ab248561 has not yet been referenced specifically in any publications.

    Publishing research using ab248561? Please let us know so that we can cite the reference in this datasheet.

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