RecombinantRabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

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Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
  • Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6148] to HLA Class II DRB1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free
    See all HLA Class II DRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
    Database link: P01911

  • Positive control

    • IHC-P: human tonsil tissue. ICC/IF: Raji cells.

  • General notes

    ab248561 is the carrier-free version of ab133578 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248561 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6148

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab248561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)
    • Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
      Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248561? Please let us know so that we can cite the reference in this datasheet.

    ab248561 has not yet been referenced specifically in any publications.

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