RecombinantRabMAb

Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

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Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
  • Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6148] to HLA Class II DRB1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free
    See all HLA Class II DRB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6148] to HLA Class II DRB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human tonsil tissue. ICC/IF: Raji cells.

  • General notes

    ab248561 is the carrier-free version of ab133578.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 8.80 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6148

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
    Immunocytochemistry/ Immunofluorescence - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
    Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133578)
  • Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)
    Anti-HLA Class II DRB1 antibody [EPR6148] - BSA and Azide free (ab248561)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab248561? Please let us know so that we can cite the reference in this datasheet.

ab248561 has not yet been referenced specifically in any publications.

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