Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [HLA-DRB/1067]
    See all HLA Class II DRB1 primary antibodies
  • Description

    Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1
  • Host species

    Mouse
  • Specificity

    Reacts with the beta chain.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class II DRB1. Activated human peripheral blood mononuclear cells.
    Database link: P01911

  • Positive control

    • Ramos cell lysate; human tonsil and histiochytoma tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab215835 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    HLA-DRB/1067
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling HLA Class II DRB1 with ab215835 at 1 μg/ml dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human histiocytoma tissue labeling HLA Class II DRB1 with ab215835 at 1 μg/ml dilution.

  • Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] (ab215835) at 1 µg/ml + Ramos cell lysate

    Predicted band size: 29 kDa

References

ab215835 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215835.
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