Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free (ab212448)
Key features and details
- Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free
See all HLA Class II DRB1 primary antibodies -
Description
Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1 - BSA and Azide free -
Host species
Mouse -
Specificity
ab212448 does not cross react with HLA-DP and HLA-DQ. ab212448 epitope is different from that of ab212449 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human HLA Class II DRB1. Activated Human peripheral blood mononuclear cells.
Database link: P01911 -
Positive control
- Ramos, Daudi, and HuT78 cells. Human tonsil, lymph node and histiocytoma tissues. Ramos cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
HLA-DRB/1067 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
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IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa. |
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 3123 Human
- Omim: 142857 Human
- SwissProt: P01911 Human
- SwissProt: P01912 Human
- SwissProt: P04229 Human
- SwissProt: P20039 Human
- SwissProt: Q29974 Human
- SwissProt: Q30134 Human
see all -
Alternative names
- 2B1F_HUMAN antibody
- DR1 antibody
- DR16 antibody
see all
Images
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Immunohistochemical analysis of formalix-fixed paraffin-embedded human tonsil tissue, labeling HLA Class II DRB1 using ab212448 at 0.5 µg/mL.
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Immunohistochemical analysis of formalix-fixed paraffin-embedded human histiocytoma tissue, labeling HLA Class II DRB1 using ab212448 at 0.5 µg/mL.
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Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free (ab212448) at 1 µg/ml + Ramos cell lysate
Predicted band size: 30 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab212448 has not yet been referenced specifically in any publications.