Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free (ab212448)

Overview

  • Product name

    Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free
    See all HLA Class II DRB1 primary antibodies
  • Description

    Mouse monoclonal [HLA-DRB/1067] to HLA Class II DRB1 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab212448 does not cross react with HLA-DP and HLA-DQ. ab212448 epitope is different from that of ab212449
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA Class II DRB1. Activated Human peripheral blood mononuclear cells.
    Database link: P01911

  • Positive control

    • Ramos, Daudi, and HuT78 cells. Human tonsil, lymph node and histiocytoma tissues. Ramos cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    HLA-DRB/1067
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 30 kDa.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalix-fixed paraffin-embedded human tonsil tissue, labeling HLA Class II DRB1 using ab212448 at 0.5 µg/mL.

  • Immunohistochemical analysis of formalix-fixed paraffin-embedded human histiocytoma tissue, labeling HLA Class II DRB1 using ab212448 at 0.5 µg/mL.

  • Anti-HLA Class II DRB1 antibody [HLA-DRB/1067] - BSA and Azide free (ab212448) at 1 µg/ml + Ramos cell lysate

    Predicted band size: 30 kDa

References

ab212448 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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