Overview

  • Product name

    Anti-HLA-DP antibody [B7/21] - BSA and Azide free
    See all HLA-DP primary antibodies
  • Description

    Mouse monoclonal [B7/21] to HLA-DP - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • IF/ICC: Raw 246.7 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    This product is a PBS-only buffer formulated version of ab20897

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    B7/21
  • Isotype

    IgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Relevance

    HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
  • Cellular localization

    Type I membrane protein; Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • Database links

  • Alternative names

    • DP(W3) antibody
    • DP(W4) antibody
    • HLA class II histocompatibility antigen DP alpha 1 chain antibody
    • HLA class II histocompatibility antigen DP alpha chain antibody
    • HLA DP1A antibody
    • HLA DPA 1 antibody
    • HLA SB alpha chain antibody
    • HLADP antibody
    • HLADPA1 antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha gene antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • MHC class II antigen antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA DPA1 antigen antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    see all

Images

  • The ICC/IF data shown was generated using the same antibody clone in a different buffer formulation (ab20897). 

    Raw 246.7 cells stained for HLA-DP (colored green) using ab20897 in ICC/IF. The cells were fixed with 4% formaldehyde (10 min) then permeabilized using 0.1% Triton X-100 in PBS buffer and then incubated in 1% bovine serum albumin (BSA) + 10% normal goat serum + 0.3 M glycine + 0.1% Tween-20 in PBS buffer for 1 hour to further permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the HLA-DP antibody ab20897 at 5 µg/ml overnight at +4°C. The secondary antibody (colored green) was Alexa Fluor® 488 goat anti-mouse (ab150117) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (colored red) at a 1/200 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1 hour at room temperature. 

References

ab222780 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222780.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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