Recombinant
RabMAb

Recombinant Anti-HLA-DP antibody [SP228] (ab227675)

Overview

  • Product name

    Anti-HLA-DP antibody [SP228]
    See all HLA-DP primary antibodies
  • Description

    Rabbit monoclonal [SP228] to HLA-DP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HLA-DP aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P20036

  • Positive control

    • IHC-P: Human tonsil tissue. Flow Cyt: Ramos cells. ICC/IF: Ramos cells.
  • General notes

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP228
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/270.
IHC-P 1/100.

Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

ICC/IF 1/25.

Target

  • Relevance

    HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
  • Cellular localization

    Type I membrane protein; Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
  • Database links

  • Alternative names

    • DP(W3) antibody
    • DP(W4) antibody
    • HLA class II histocompatibility antigen DP alpha 1 chain antibody
    • HLA class II histocompatibility antigen DP alpha chain antibody
    • HLA DP1A antibody
    • HLA DPA 1 antibody
    • HLA SB alpha chain antibody
    • HLADP antibody
    • HLADPA1 antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha gene antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • MHC class II antigen antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA DPA1 antigen antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DP with ab227675 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (11 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DP with ab227675 (green) compared with a rabbit isotype control (blue).

  • Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

References

ab227675 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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