Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP228] to HLA-DP
- Suitable for: Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-HLA-DP antibody [SP228]
See all HLA-DP primary antibodies
DescriptionRabbit monoclonal [SP228] to HLA-DP
Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HLA-DP aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P20036
- IHC-P: Human tonsil tissue. Flow Cyt: Ramos cells. ICC/IF: Ramos cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
RelevanceHLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
Cellular localizationType I membrane protein; Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
- DP(W3) antibody
- DP(W4) antibody
- HLA class II histocompatibility antigen DP alpha 1 chain antibody
Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DP with ab227675 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (11 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DP with ab227675 (green) compared with a rabbit isotype control (blue).
Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DP with purified ab227675 (1.02 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227675 has not yet been referenced specifically in any publications.