Overview

  • Product name
    Anti-HLA-DQ antibody [SPV-L3]
    See all HLA-DQ primary antibodies
  • Description
    Mouse monoclonal [SPV-L3] to HLA-DQ
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, Inhibition Assay, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human, Pig
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA-DQ.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ion Exchange Chromatography
  • Clonality
    Monoclonal
  • Clone number
    SPV-L3
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. PubMed: 15795275

Removal of sodium azide is recommended prior to use in functional assays.

Target

  • Relevance
    Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens: class I (HLA A, HLA B and HLA C) and class II (HLA D).
  • Cellular localization
    Type I membrane protein
  • Database links
  • Alternative names
    • CD antibody
    • CELIAC1 antibody
    • DC 4 alpha chain antibody
    • DC alpha antibody
    • DQ A1 antibody
    • Gluten sensitive enteropathy (celiac disease) antibody
    • GSE antibody
    • HLA Class II DQ antibody
    • HLA class II histocompatibility antigen DQ antibody
    • HLA class II histocompatibility antigen DQ(1) alpha chain antibody
    • HLA class II histocompatibility antigen DQ(3) alpha chain antibody
    • HLA DCA antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Leucocyte antigen DQA1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • MHC class II antigen HLA DQ beta 1 antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DQ beta glycoprotein antibody
    • MHC DQ beta antibody
    • MHC HLA DQ alpha antibody
    see all

References

This product has been referenced in:
  • Hirayama M  et al. An oncofetal antigen, IMP-3-derived long peptides induce immune responses of both helper T cells and CTLs. Oncoimmunology 5:e1123368 (2016). Read more (PubMed: 27471607) »
  • Fairfax BP  et al. Innate immune activity conditions the effect of regulatory variants upon monocyte gene expression. Science 343:1246949 (2014). Flow Cyt ; Human . Read more (PubMed: 24604202) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you very much for your reply.

The binding affinity and mechanism varies between blocking antibodies, butthey do seem to generally act as antagonists. These kinds of characteristics need to be determined for each individual antibody and antigen. For the HLA antibody Ab23632, we only have one publication demonstrating its use in a blocking experiment and there doesn't seem to be any data regarding the binding affinity of the antibody-

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069546/

The specific time the antibody will bind to a single antigen depends on factors of the experiment such as temperature, concentration of the antibody in the media, cell type, etc. I wouldn't expect the blocking to be effective for many days, but probably the effect will be noticeable on the hour-scale.

I am sorry that we don't havemore information regarding this, but if you have further questions or need anything else, please let me know and I'll be happy to help.

Read More

Answer

Thank you again for contacting us and for your patience while I've been in touch with the lab about your enquiry.

Ab20897 against HLA-DP has not been tested in a blocking experiment at this time, though it is possible that it will work in such an assay. Unfortunately none of our other HLA-DP antibodies seem tohave been tested for blocking ability atthis time. If you're interested in trying Ab20897 in a blocking experiment, we do have a testing discount programand I'd be happy to send more information about this if you're interested.

The clone SPV-L3 against HLA-DQ, which we carry as Ab23632, has been used in a blocking experiment in the following publication-

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069546/

We would recommend removing the sodium azide from Ab23632 before incubating it with live cells, as azide will cause cell death. An antibody purification kit such as Ab102784 can be used to remove the azide-

https://www.abcam.com/Antibody-Purification-Kit-Protein-A-ab102784.html

I hope that this information is useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Answer

Thank you very much for contacting us with your enquiry.

Theterm "functional studies" can be used to describe several different kinds of assays, and sometimes blocking assays are included in this categorization so there isn't a specific difference between blocking and functional studies antibodies.

For example, ab136320 is listed as being tested in functional studies and a blocking assay, however after checking through our internal notes I see that the functional study was actually a blocking assay. Ab23755 was also tested in a functional study, however this was a tissue typing and crossmatching experiment,not a blocking assay. The details of the functional study are often on the datasheet in the "Application Notes" section, however this is not always the case. I apologize that these designationsare not more straight-forward and clear.

Are you using human samples? Ab136320 will be a good antibody for blocking HLA DR, but we don't currently have any antibodies listed as being tested for blocking of HLA DP and HLA DQ. There are two antibodies to these targets that might work in such an assay, ab20897 (HLA DP) and ab23632 (HLA DQ), but I'm checking with the lab to make sure they've been tested for blocking ability. I'll get back to you as soon as I have confirmed this.

I hope that this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Answer

Thank you for your enquiry. This product was produced by immunising mice with the HG-38 Cell line. Our QC lab uses either western blot or flowcytometry to determine specificity. Finally, this clone SPV-L3 has been reported to inhibit the activity of cytotoxic T cell clones. Spits, H. et al. (1984) Eur. J. Immunol. 14: 299-304. Gudgeon, N et al. (2005) J. Virology. 79:5477-5488. I hope this information will be useful.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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