Overview

  • Product name
  • Description
    Rabbit polyclonal to HLA-DQA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HLA-DQA1 aa 56-84 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P01909

  • Positive control
    • K562 cell lysate; K562 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170578 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities
    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD antibody
    • CELIAC1 antibody
    • DC 1 alpha chain antibody
    • DC alpha antibody
    • DC-1 alpha chain antibody
    • DC-alpha antibody
    • DC1, included antibody
    • DQ alpha 1 chain antibody
    • DQ-A1 antibody
    • DQ-DRW9 alpha chain antibody
    • DQA1_HUMAN antibody
    • FLJ27088 antibody
    • FLJ27328 antibody
    • Gluten-sensitive enteropathy (celiac disease) antibody
    • GSE antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen, DQ alpha 1 chain antibody
    • HLA class II histocompatibility antigen, DQ(W3) alpha chain antibody
    • HLA-DCA antibody
    • HLA-DQA antibody
    • HLA-DQA1 antibody
    • HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 antibody
    • HLADC histocompatibility type antibody
    • Immune response antigens HIa, included antibody
    • leucocyte antigen DQA1 antibody
    • leukocyte antigen alpha chain antibody
    • LOC100133678 antibody
    • LOC100507686 antibody
    • LOC100509457 antibody
    • Major histocompatibility complex, class II, DQ alpha 1 antibody
    • MGC149527 antibody
    • MHC class II antigen antibody
    • MHC class II DQA1 antibody
    • MHC class II HLA-D alpha glycoprotein antibody
    • MHC class II HLA-DQ alpha 1 antibody
    • MHC class II surface glycoprotein antibody
    • MHC HLA-DQ alpha antibody
    • OTTHUMP00000029141 antibody
    • OTTHUMP00000176885 antibody
    • OTTHUMP00000178551 antibody
    • OTTHUMP00000178552 antibody
    • OTTHUMP00000233817 antibody
    see all

Images

  • Flow cytometric analysis of K562 cells labeling HLA-DQA1with ab170578 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-HLA-DQA1 antibody (ab170578) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 28 kDa

References

ab170578 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170578.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up