Overview

  • Product name

    Anti-HLA-DQA1 antibody [EPR7300]
    See all HLA-DQA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7300] to HLA-DQA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HLA-DQA1 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human lymph node, spleen, tonsil, Daudi, and Raji cell lysates; Human colon and tonsil tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 33-35 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • Sequence similarities

      Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD antibody
      • CELIAC1 antibody
      • DC 1 alpha chain antibody
      • DC alpha antibody
      • DC-1 alpha chain antibody
      • DC-alpha antibody
      • DC1, included antibody
      • DQ alpha 1 chain antibody
      • DQ-A1 antibody
      • DQ-DRW9 alpha chain antibody
      • DQA1_HUMAN antibody
      • FLJ27088 antibody
      • FLJ27328 antibody
      • Gluten-sensitive enteropathy (celiac disease) antibody
      • GSE antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen, DQ alpha 1 chain antibody
      • HLA class II histocompatibility antigen, DQ(W3) alpha chain antibody
      • HLA-DCA antibody
      • HLA-DQA antibody
      • HLA-DQA1 antibody
      • HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 antibody
      • HLADC histocompatibility type antibody
      • Immune response antigens HIa, included antibody
      • leucocyte antigen DQA1 antibody
      • leukocyte antigen alpha chain antibody
      • LOC100133678 antibody
      • LOC100507686 antibody
      • LOC100509457 antibody
      • Major histocompatibility complex, class II, DQ alpha 1 antibody
      • MGC149527 antibody
      • MHC class II antigen antibody
      • MHC class II DQA1 antibody
      • MHC class II HLA-D alpha glycoprotein antibody
      • MHC class II HLA-DQ alpha 1 antibody
      • MHC class II surface glycoprotein antibody
      • MHC HLA-DQ alpha antibody
      • OTTHUMP00000029141 antibody
      • OTTHUMP00000176885 antibody
      • OTTHUMP00000178551 antibody
      • OTTHUMP00000178552 antibody
      • OTTHUMP00000233817 antibody
      see all

    Images

    • ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-HLA-DQA1 antibody [EPR7300] (ab128959) at 1/10000 dilution

      Lane 1 : Human lymph node lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Human spleen lysate
      Lane 4 : Raji cell lysate
      Lane 5 : Human tonsil lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 33-35 kDa
      why is the actual band size different from the predicted?

    • ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Kanangat S  et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . Read more (PubMed: 29656111) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 30 min 95C
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted May 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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