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Immunology Adaptive Immunity MHC Class II
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Anti-HLA-DQA2 antibody (ab136667)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA2 antibody (ab136667)

    Key features and details

    • Goat polyclonal to HLA-DQA2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

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    Overview

    • Product name

      Anti-HLA-DQA2 antibody
    • Description

      Goat polyclonal to HLA-DQA2
    • Host species

      Goat
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Sheep, Goat, Cow, Chimpanzee, Gorilla
    • Immunogen

      Synthetic peptide:

      C-QSHGPSGQYTH

      , corresponding to internal sequence amino acids 40-50 of Human HLA-DQA2 (NP_064440.1).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human spleen tissue
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, 99.48% Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • MHC
      • Class II

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab136667 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    Target

    • Function

      Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • Tissue specificity

      Expressed at low levels at the surface of B lymphoblastoid cells.
    • Sequence similarities

      Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Target information above from: UniProt accession P01906 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3118 Human
      • Omim: 613503 Human
      • SwissProt: P01906 Human
      • Unigene: 591798 Human
      • Alternative names

        • DQ(6) alpha chain antibody
        • DQA2_HUMAN antibody
        • DX alpha antibody
        • DX alpha chain antibody
        • HLA class II histocompatibility antigen antibody
        • HLA class II histocompatibility antigen DQ(6) alpha chain antibody
        • HLA class II histocompatibility antigen, DQ alpha 2 chain antibody
        • HLA DXA antibody
        • HLA-DQA1 antibody
        • HLA-DQA2 antibody
        • Major histocompatibility complex class II DQ alpha 2 antibody
        • MHC class II DQA2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA2 antibody (ab136667)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA2 antibody (ab136667)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labelling HLA-DQA2 with ab136667 at 3.75µg/ml after heat-induced antigen retrieval.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab136667? Please let us know so that we can cite the reference in this datasheet.

      ab136667 has not yet been referenced specifically in any publications.

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