Anti-HLA-DQB2 antibody (ab126139)

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Overview

  • Product name

    Anti-HLA-DQB2 antibody

  • Description

    Rabbit polyclonal to HLA-DQB2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-195 of HLA-DQB2

  • Positive control

    • K562 whole cell lysate; Human colon carcinoma tissue.

  • General notes

    Store as concentrated solution.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Belongs to the MHC class II family. Contains one Ig like C1 type (immunoglobulin like) domain.

  • Cellular localization

    Cell membrane; Single pass type I membrane protein

  • Database links

    • Alternative names

      • DV19.1 (major histocompatibility complex, class II, DQ beta 2 (HLA-DXB)) antibody
      • DX-beta antibody
      • HLA class II histocompatibility antigen, DQ beta 2 chain antibody
      • HLA class II histocompatibility antigen, DX beta chain antibody
      • HLA DQB1 antibody
      • HLA DXB antibody
      • major histocompatibility complex, class II, DQ beta 1 antibody
      • Major histocompatibility complex, class II, DQ beta 2 antibody
      • Major histocompatibility complex, class II, DX beta antibody
      • MHC class II antigen DQB2 antibody
      see all

    Images

    • Western blot - Anti-HLA-DQB2 antibody (ab126139)
      Western blot - Anti-HLA-DQB2 antibody (ab126139)
      Anti-HLA-DQB2 antibody (ab126139) at 1/1000 dilution + K562 lysate at 30 µg

      Predicted band size: 30 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQB2 antibody (ab126139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQB2 antibody (ab126139)
      ab126139, at 1/500 dilution, staining HLA-DQB2 in Formalin-fixed, Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

    References

    ab126139 has not yet been referenced specifically in any publications.

    Publishing research using ab126139? Please let us know so that we can cite the reference in this datasheet.

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