Overview

  • Product name
    Anti-HLA-DQB2 antibody
    See all HLA-DQB2 primary antibodies
  • Description
    Rabbit polyclonal to HLA-DQB2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-195 of HLA-DQB2

  • Positive control
    • K562 whole cell lysate; Human colon carcinoma tissue.
  • General notes
    Store as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Belongs to the MHC class II family. Contains one Ig like C1 type (immunoglobulin like) domain.
  • Cellular localization
    Cell membrane; Single pass type I membrane protein
  • Database links
    • Alternative names
      • DV19.1 (major histocompatibility complex, class II, DQ beta 2 (HLA-DXB)) antibody
      • DX-beta antibody
      • HLA class II histocompatibility antigen, DQ beta 2 chain antibody
      • HLA class II histocompatibility antigen, DX beta chain antibody
      • HLA DQB1 antibody
      • HLA DXB antibody
      • major histocompatibility complex, class II, DQ beta 1 antibody
      • Major histocompatibility complex, class II, DQ beta 2 antibody
      • Major histocompatibility complex, class II, DX beta antibody
      • MHC class II antigen DQB2 antibody
      see all

    Images

    • Western blot - Anti-HLA-DQB2 antibody (ab126139)
      Western blot - Anti-HLA-DQB2 antibody (ab126139)
      Anti-HLA-DQB2 antibody (ab126139) at 1/1000 dilution + K562 lysate at 30 µg

      Predicted band size: 30 kDa



      12% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQB2 antibody (ab126139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQB2 antibody (ab126139)
      ab126139, at 1/500 dilution, staining HLA-DQB2 in Formalin-fixed, Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.

    References

    ab126139 has not yet been referenced specifically in any publications.

    Publishing research using ab126139? Please let us know so that we can cite the reference in this datasheet.

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