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    hla-dr--dp--dq-antibody-cr343-ab7856.pdf

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Immunology Adaptive Immunity MHC Class II
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Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)

  • Datasheet
  • SDS
Reviews (3)Q&A (7)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)

Key features and details

  • Mouse monoclonal [CR3/43] to HLA DR + DP + DQ
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-HLA DR + DP + DQ antibody [CR3/43]
    See all HLA DR + DP + DQ primary antibodies
  • Description

    Mouse monoclonal [CR3/43] to HLA DR + DP + DQ
  • Host species

    Mouse
  • Specificity

    The gene of the human histocompatibility complex class II consists of at least four subregions: HLA DP, DQ, and DR, containing a minimum of one alpha and one beta chain. This antibody reacts with the beta-chain of all products of the gene subregions DP, DQ, and DR.

  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human HLA DR + DP + DQ. Raised against tonsil cells of human origin

  • Positive control

    • IHC-P: Human tonsil tissue
  • General notes


    This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Primary antibody notes

    This antibody principally labels B cells, interdigitating reticulum cells, Langerhans’ cells and manymacrophages.
  • Clonality

    Monoclonal
  • Clone number

    CR3/43
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

Our Abpromise guarantee covers the use of ab7856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500. Detects a band of approximately 30 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Target information above from: UniProt accession P04440 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3115 Human
    • Entrez Gene: 3117 Human
    • Entrez Gene: 3119 Human
    • Entrez Gene: 3122 Human
    • Entrez Gene: 3123 Human
    • Entrez Gene: 3125 Human
    • Entrez Gene: 3126 Human
    • Entrez Gene: 3127 Human
    • Omim: 142858 Human
    • Omim: 142860 Human
    • Omim: 142880 Human
    • Omim: 146880 Human
    • SwissProt: P01903 Human
    • SwissProt: P01913 Human
    • SwissProt: P04440 Human
    • SwissProt: P79483 Human
    • SwissProt: Q30631 Human
    • Unigene: 485130 Human
    see all
  • Alternative names

    • CD antibody
    • CELIAC 1 antibody
    • CELIAC1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DQ A1 antibody
    • DRB1 antibody
    • DRB4 antibody
    • FLJ27088 antibody
    • FLJ27328 antibody
    • GSE antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR 1 beta antibody
    • HLA class II histocompatibility antigen DR alpha antibody
    • HLA class II histocompatibility antigen DR alpha chain antibody
    • HLA DP histocompatibility type beta 1 subunit antibody
    • HLA DP1B antibody
    • HLA DPB1 antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • HLA DR1B antibody
    • HLA DR3B antibody
    • HLA DR4B antibody
    • HLA DRA antibody
    • HLA DRA1 antibody
    • HLA DRB1 antibody
    • HLA DRB3 antibody
    • HLA DRB4 antibody
    • HLA DRB5 antibody
    • HLA-DPB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • Major histocompatibility complex class II DR alpha antibody
    • Major histocompatibility complex class II DR beta 1 antibody
    • Major histocompatibility complex class II DR beta 3 antibody
    • Major histocompatibility complex class II DR beta 4 antibody
    • Major histocompatibility complex class II DR beta 5 antibody
    • MGC117330 antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II antigen DRA antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DR beta 1 antibody
    • MHC class II HLA DR beta 3 antibody
    • MHC DPB1 antibody
    • MHC DQ beta antibody
    • MHC HLA DPB1 antibody
    • MHC HLA DQ alpha antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)

     IHC image of -HLA DR + DP + DQ staining in a section of formalin-fixed paraffin-embedded normal human tonsil* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab7856, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    IHC image of ab7856 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7856, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Western blot - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    All lanes : Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856) at 1/500 dilution

    Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 27 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA DR + DP + DQ antibody [CR3/43] (ab7856)This image is courtesy of an anonymous Abreview

    Ab7856 staining HLA DR+DP+DQ in Human Colorectal tissue sections by Immunohistochemistry. Antigen retrieval was by heat mediation at pH6 for 20minutes.  Samples were fixed with formaldehyde and blocked with 10% hydrogen peroxide for 5 minutes. Samples were incubated with primary antibody at 1/100 dilution for 15 minutes. HRP rabbit polyclonal polymer refine detection kit was used as the secondary antibody.

    See Abreview

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab7856? Please let us know so that we can cite the reference in this datasheet.

    ab7856 has been referenced in 6 publications.

    • Campbell MJ  et al. Characterizing the immune microenvironment in high-risk ductal carcinoma in situ of the breast. Breast Cancer Res Treat 161:17-28 (2017). PubMed: 27785654
    • Pandya H  et al. Differentiation of human and murine induced pluripotent stem cells to microglia-like cells. Nat Neurosci 20:753-759 (2017). PubMed: 28253233
    • Peng W  et al. Distinct Expression and Function of FceRII in Human B Cells and Monocytes. J Immunol 198:3033-3044 (2017). PubMed: 28275138
    • Jayachandran A  et al. Embryonic Chicken Transplantation is a Promising Model for Studying the Invasive Behavior of Melanoma Cells. Front Oncol 5:36 (2015). PubMed: 25763357
    • Breshears LM  et al. A Disintegrin and Metalloproteinase 17 (ADAM17) and Epidermal Growth Factor Receptor (EGFR) Signaling Drive the Epithelial Response to Staphylococcus aureus Toxic Shock Syndrome Toxin-1 (TSST-1). J Biol Chem 287:32578-87 (2012). WB . PubMed: 22833676
    • Shaikh SB & Nicholson LF Effects of chronic low dose rotenone treatment on human microglial cells. Mol Neurodegener 4:55 (2009). PubMed: 20042120

    Customer reviews and Q&As

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    1-10 of 10 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Tonsil
    Blocking step
    Dako Protein Block X0909 as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Mrs. Amanda Burnette

    Verified customer

    Submitted Dec 20 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR + DP + DQ antibody [CR3/43]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colorectal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6 20 minutes
    Permeabilization
    No
    Specification
    Colorectal
    Blocking step
    hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 10%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 16 2016

    Question

    What is the concentration of this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 16 2012

    Answer

    Thank you for your inquiry.

    I heard back from our lab that the concentration of all lots of ab7856 is 0.2 mg/ml. I have since added this information to the datasheet, but it may take a few days for the system to show it.

    I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Apr 16 2012

    Question

     Hello, This antibody is no longer available. Which one would you recommand instead? Is there a publication showing it has already been used for immunohistochemistry on skin? Thank you in advance. Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2011

    Answer

    Thank you for contacting us. The clone CR3/43 has already been used frequently in immunohistochemistry. I have no acces to some articles, but I think it has been used in here for skin staining: https://springerlink3.metapress.com/content/x6801633p4761hh1/resource-secured/?target=fulltext.pdf&sid=zsvhv2pbyiz1baud0nolf5c4&sh=www.springerlink.com and http://www.liebertonline.com/doi/abs/10.1089/thy.1996.6.41 Even without image, the clone has also been used in this article: http://www.springerlink.com/content/gw57180081qulj62/ We do guarantee our products to work as stated on the datasheet, and do therefore guarantee the anti HLA DR+DP+DQ antibodies, clone CR3/43, ab7856 and the ab17076 (prediluted) in immunohistochemistry on human paraffin sections (the ab17076 also on frozen sections). https://www.abcam.com/index.html?datasheet=7856 https://www.abcam.com/index.html?datasheet=17076 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2011

    Question

    I could not find the concentration of this antibody, What is it?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 04 2005

    Answer

    Thank you for your enquiry. We cannot provide you with a concentration for this antibody, as it is not a purified antibody. Please contact us again if you require further assistance.

    Read More

    Abcam Scientific Support

    Answered on Feb 04 2005

    Question

    ANTIBODY STORAGE CONDITIONS Our customer would like to kow if there is a specific antigen retrieval protocol needed for this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 12 2003

    Answer

    There is no specific antigen retrieval step stated on the antibody data sheet, so we recommend for Immunohistochemistry (Formalin-fixed paraffin-embedded sections)starting off by trying the heat mediated pressure cooker method.

    Read More

    Abcam Scientific Support

    Answered on Dec 12 2003

    Question

    I wanted to ask you whether you have the unconjugated CR3/43 antibody without Na-azide and what would be in this case the price of this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 08 2003

    Answer

    Thank you for your enquiry. Ab7856 is only available as described on the online datasheet. To see if another company may offer a CR3/43 antibody without Na azide, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

    Read More

    Abcam Scientific Support

    Answered on Dec 08 2003

    Question

    Do you know if this ab is cross-reactive with non-human primates, specifically rhesus monkeys?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We are very sorry but there is no information about the cross-reactivity with rhesus monkeys. All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). We will update the on-line datasheet of this product as soon as get more data.

    Read More

    Asdf Edo

    Abcam Scientific Support

    Answered on Oct 13 2003

    Question

    Do you have a similar polyspecific antibody (DR,DP.DQ) appropriate for flow cytometry?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 03 2001

    Answer

    Although this antibody has not been specifically tested in flow cytometry, we would expect it to work well in that application. Abcam does have other antibodies to HLA-DR available, however they are monospecific.

    Read More

    Abcam Scientific Support

    Answered on Oct 04 2001

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    For licensing inquiries, please contact partnerships@abcam.com

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