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Inquiry: I am doing a western blot. The whole cell lysate is human B cell line. The antibody that I want is anti MHC II. I tried L243 which is DR, it did not work for western blot. Now I can not decide between your ab124809, ab118347(both DRA1) and 116378( DP). Which one do you recommend for western blot? Also, above antibodies' price are so high. What if I only need to try one time, it may or may not fit my experiment for the future. Do you have any small size, trial sample or discount of some kinds?
Asked on Jul 02 2012
Thank you for contacting us.
I assume you were refering to ab107604 as the L243 antibody (https://www.abcam.com/hla-dr-antibody-l243-phycoerythrin-ab107604.html)? This antibody has not been tested in WB yet and is PE-conjugated. Please feel free to submit an Abreview with your WB data and earn Abpoints.
As for the other 3 antibodies:
If you are looking for an MHC CLass II antibody, ab116378 would be the best choice. The other 2 are HLA DR specific. All three were tested in WBwith human samples and would therefore be guaranteed. But it depends on the target protein of your interest, which one would be the better choice for you.
We do not offer sample sizes, butguarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise
We have promotions which can be found here: https://www.abcam.com/promotions
Discount applies when purchasing 5 vials or more. Please feel free to check with our customer service when placing the order for any current updates on promotions.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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Answered on Jul 02 2012