Overview

  • Product name

  • Description

    Rabbit polyclonal to HLA-DR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rabbit
  • Immunogen

    Recombinant fragment corresponding to Rabbit HLA-DR aa 26-211. (NM_001171118.1). (Expressed in E.coli).
    Sequence:

    AIKEEHVIIQAEYFLSPDDLGEFMFDFDGDEIFHVDMDKKETVWRLKEFG QFASFEAQGALANIAVDRANLDIMIKRSNHTPDTNVPPEVTLLPSSPVEL GEPNVLICFIDKFSPPVINVTWLRNGNPVTMGVSETVFLPRDDHLFRKFH YLPFLPSTEDVYDCKVEHWGLEEPTLKHWEFEARTPLPETTENVVC

  • Positive control

    • WB: Rabbit spleen, lymph and thymus lysates; Recombinant rabbit HLA-DR protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Followed by protein A chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab231004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 29 kDa.

Target

  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Alternative names

    • DASS-397D15.1 antibody
    • DR alpha chain antibody
    • DR alpha chain precursor antibody
    • DRA_HUMAN antibody
    • DRB1 antibody
    • DRB4 antibody
    • FLJ51114 antibody
    • Histocompatibility antigen HLA DR alpha antibody
    • Histocompatibility antigen HLA-DR alpha antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR alpha chain antibody
    • HLA DR1B antibody
    • HLA DR3B antibody
    • HLA DRA antibody
    • HLA DRA1 antibody
    • HLA DRB1 antibody
    • HLA DRB3 antibody
    • HLA DRB4 antibody
    • HLA DRB5 antibody
    • HLA-DR histocompatibility type antibody
    • HLA-DRA antibody
    • HLADR4B antibody
    • HLADRA1 antibody
    • HLADRB antibody
    • Major histocompatibility complex class II DR alpha antibody
    • Major histocompatibility complex class II DR beta 1 antibody
    • Major histocompatibility complex class II DR beta 3 antibody
    • Major histocompatibility complex class II DR beta 4 antibody
    • Major histocompatibility complex class II DR beta 5 antibody
    • MGC117330 antibody
    • MHC cell surface glycoprotein antibody
    • MHC class II antigen DRA antibody
    • MHC II antibody
    • MLRW antibody
    • OTTHUMP00000029406 antibody
    • OTTHUMP00000029407 antibody
    see all

Images

  • All lanes : Anti-HLA-DR antibody (ab231004) at 3 µg/ml

    Lane 1 : Rabbit spleen tissue
    Lane 2 : Rabbit lymph tissue
    Lane 3 : Rabbit thymus tissue

    Secondary
    All lanes : HRP-Linked Rabbit Anti-Guinea IgG antibody at 1/5000 dilution

    Predicted band size: 29 kDa

  • Anti-HLA-DR antibody (ab231004) at 3 µg/ml + Recombinant MHCDRa protein

    Secondary
    HRP-Linked Rabbit Anti-Guinea IgG antibody at 1/5000 dilution

    Predicted band size: 29 kDa

References

ab231004 has not yet been referenced specifically in any publications.

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