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Immunology Adaptive Immunity MHC Class II
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RecombinantRabMAb

Recombinant Anti-HLA-DR antibody [EPR3691(2)] (ab124809)

  • Datasheet
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Western blot - Anti-HLA-DR antibody [EPR3691(2)] (ab124809)
  • Anti-HLA-DR antibody [EPR3691(2)] (ab124809)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3691(2)] to HLA-DR
  • Suitable for: WB
  • Reacts with: Human

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Protein
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Recombinant Human HLA-DR protein (ab177661)
Secondary
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Overview

  • Product name

    Anti-HLA-DR antibody [EPR3691(2)]
    See all HLA-DR primary antibodies
  • Description

    Rabbit monoclonal [EPR3691(2)] to HLA-DR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human HLA-DR aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Raji, Ramos, HuT-78 and Human spleen lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3691(2)
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Associated products

  • Alternative Versions

    • Anti-HLA-DR antibody [EPR3691(2)] - BSA and Azide free (ab248013)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Raji membrane extract lysate (ab30126)
    • Ramos whole cell lysate (ab3955)
  • Recombinant Protein

    • Recombinant Human HLA-DR protein (ab177661)

Applications

Our Abpromise guarantee covers the use of ab124809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • Sequence similarities

      Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
    • Cellular localization

      Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Target information above from: UniProt accession P01903 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3122 Human
      • Omim: 142860 Human
      • SwissProt: P01903 Human
      • Unigene: 520048 Human
      • Alternative names

        • DASS-397D15.1 antibody
        • DR alpha chain antibody
        • DR alpha chain precursor antibody
        • DRA_HUMAN antibody
        • DRB1 antibody
        • DRB4 antibody
        • FLJ51114 antibody
        • Histocompatibility antigen HLA DR alpha antibody
        • Histocompatibility antigen HLA-DR alpha antibody
        • HLA class II histocompatibility antigen antibody
        • HLA class II histocompatibility antigen DR alpha chain antibody
        • HLA DR1B antibody
        • HLA DR3B antibody
        • HLA DRA antibody
        • HLA DRA1 antibody
        • HLA DRB1 antibody
        • HLA DRB3 antibody
        • HLA DRB4 antibody
        • HLA DRB5 antibody
        • HLA-DR histocompatibility type antibody
        • HLA-DRA antibody
        • HLADR4B antibody
        • HLADRA1 antibody
        • HLADRB antibody
        • Major histocompatibility complex class II DR alpha antibody
        • Major histocompatibility complex class II DR beta 1 antibody
        • Major histocompatibility complex class II DR beta 3 antibody
        • Major histocompatibility complex class II DR beta 4 antibody
        • Major histocompatibility complex class II DR beta 5 antibody
        • MGC117330 antibody
        • MHC cell surface glycoprotein antibody
        • MHC class II antigen DRA antibody
        • MHC II antibody
        • MLRW antibody
        • OTTHUMP00000029406 antibody
        • OTTHUMP00000029407 antibody
        see all

      Images

      • Western blot - Anti-HLA-DR antibody [EPR3691(2)] (ab124809)
        Western blot - Anti-HLA-DR antibody [EPR3691(2)] (ab124809)
        All lanes : Anti-HLA-DR antibody [EPR3691(2)] (ab124809) at 1/1000 dilution

        Lane 1 : Raji lysate
        Lane 2 : Ramos lysate
        Lane 3 : HuT-78 lysate
        Lane 4 : Human spleen lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 29 kDa
        Observed band size: 35 kDa
        why is the actual band size different from the predicted?

      • Anti-HLA-DR antibody [EPR3691(2)] (ab124809)
        Anti-HLA-DR antibody [EPR3691(2)] (ab124809)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab124809? Please let us know so that we can cite the reference in this datasheet.

      ab124809 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Inquiry: I am doing a western blot. The whole cell lysate is human B cell line. The antibody that I want is anti MHC II. I tried L243 which is DR, it did not work for western blot. Now I can not decide between your ab124809, ab118347(both DRA1) and 116378( DP). Which one do you recommend for western blot? Also, above antibodies' price are so high. What if I only need to try one time, it may or may not fit my experiment for the future. Do you have any small size, trial sample or discount of some kinds?

      Read More

      Abcam community

      Verified customer

      Asked on Jul 02 2012

      Answer

      Thank you for contacting us.
      I assume you were refering to ab107604 as the L243 antibody (https://www.abcam.com/hla-dr-antibody-l243-phycoerythrin-ab107604.html)? This antibody has not been tested in WB yet and is PE-conjugated. Please feel free to submit an Abreview with your WB data and earn Abpoints.

      As for the other 3 antibodies:

      If you are looking for an MHC CLass II antibody, ab116378 would be the best choice. The other 2 are HLA DR specific. All three were tested in WBwith human samples and would therefore be guaranteed. But it depends on the target protein of your interest, which one would be the better choice for you.

      We do not offer sample sizes, butguarantee all our products for TESTED species and applications as listed on the datasheet. The guarantee is valid for 6 months after a purchase. For more details about our Abpromise guarantee please check this link: https://www.abcam.com/abpromise

      We have promotions which can be found here: https://www.abcam.com/promotions
      Discount applies when purchasing 5 vials or more. Please feel free to check with our customer service when placing the order for any current updates on promotions.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jul 02 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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