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  1. Link

    hla-dr-antibody-tal-1b5-ab20181.pdf

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Immunology Adaptive Immunity MHC Class II
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Anti-HLA-DR antibody [TAL 1B5] (ab20181)

  • Datasheet
  • SDS
Reviews (6)Q&A (3)References (29)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
  • Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
  • Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
  • Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
  • Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)

Key features and details

  • Mouse monoclonal [TAL 1B5] to HLA-DR
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647

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Overview

  • Product name

    Anti-HLA-DR antibody [TAL 1B5]
    See all HLA-DR primary antibodies
  • Description

    Mouse monoclonal [TAL 1B5] to HLA-DR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ab20181 gave a positive signal in Raji, Ramos and Daudi whole cell lysates, and in the following human tissue lysates: spleen; tonsil; liver. This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    TAL 1B5
  • Myeloma

    P3-NS1/1-Ag4-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class II

Associated products

  • Alternative Versions

    • Anti-HLA-DR antibody [TAL 1B5] - BSA and Azide free (ab176408)
    • Alexa Fluor® 647 Anti-HLA-DR antibody [TAL 1B5] (ab223907)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human HLA-DR protein (ab177661)
  • Related Products

    • Anti-HLA-DP antibody [B7/21] (ab20897)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 0.667 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

IHC-P (3)
Use a concentration of 0.334 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 11.117 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Notes
WB
Use a concentration of 0.667 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

IHC-P
Use a concentration of 0.334 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 11.117 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Ubiquitinated by MARCH1 or MARCH8 at Lys-244 leading to down-regulation of MHC class II. When associated with ubiquitination of the beta subunit of HLA-DR: HLA-DRB4 'Lys-254', the down-regulation of MHC class II may be highly effective.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Target information above from: UniProt accession P01903 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3122 Human
    • Omim: 142860 Human
    • SwissProt: P01903 Human
    • Unigene: 520048 Human
    • Alternative names

      • DASS-397D15.1 antibody
      • DR alpha chain antibody
      • DR alpha chain precursor antibody
      • DRA_HUMAN antibody
      • DRB1 antibody
      • DRB4 antibody
      • FLJ51114 antibody
      • Histocompatibility antigen HLA DR alpha antibody
      • Histocompatibility antigen HLA-DR alpha antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen DR alpha chain antibody
      • HLA DR1B antibody
      • HLA DR3B antibody
      • HLA DRA antibody
      • HLA DRA1 antibody
      • HLA DRB1 antibody
      • HLA DRB3 antibody
      • HLA DRB4 antibody
      • HLA DRB5 antibody
      • HLA-DR histocompatibility type antibody
      • HLA-DRA antibody
      • HLADR4B antibody
      • HLADRA1 antibody
      • HLADRB antibody
      • Major histocompatibility complex class II DR alpha antibody
      • Major histocompatibility complex class II DR beta 1 antibody
      • Major histocompatibility complex class II DR beta 3 antibody
      • Major histocompatibility complex class II DR beta 4 antibody
      • Major histocompatibility complex class II DR beta 5 antibody
      • MGC117330 antibody
      • MHC cell surface glycoprotein antibody
      • MHC class II antigen DRA antibody
      • MHC II antibody
      • MLRW antibody
      • OTTHUMP00000029406 antibody
      • OTTHUMP00000029407 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)

      IHC image of HLA-DR [TAL 1B5] staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab20181, 0.334µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      All lanes : Anti-HLA-DR antibody [TAL 1B5] (ab20181) at 0.667 µg/ml

      Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 2 : Daudi whole cell lysate (ab3951)
      Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 4 : Human spleen tissue lysate - total protein (ab29699)
      Lane 5 : Tonsil (Human) Tissue Lysate
      Lane 6 : Human liver tissue lysate - total protein (ab29889)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 29 kDa
      Observed band size: 29,35 kDa why is the actual band size different from the predicted?


      Exposure time: 16 minutes
    • Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)

      Human peripheral blood lymphocytes stained with ab20181 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab20181, 11.117µg/ml) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy – peripheral blood lymphocytes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DR antibody [TAL 1B5] (ab20181)

      Ab20181 staining human normal thymus tissue. Staining is localised to cellular membranes.
      Left panel: with primary antibody at 0.334µg/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    • Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Western blot - Anti-HLA-DR antibody [TAL 1B5] (ab20181)Image courtesy of an anonymous Abreview.
      Anti-HLA-DR antibody [TAL 1B5] (ab20181) at 0.667 µg/ml + whole tissue lysate prepared from human colon at 50 µg

      Secondary
      HRP conjugated goat anti-mouse polyclonal at 1/3000 dilution

      Developed using the ECL technique.

      Predicted band size: 29 kDa
      Observed band size: 33 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds

      See Abreview

    • Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)
      Flow Cytometry - Anti-HLA-DR antibody [TAL 1B5] (ab20181)

      Human peripheral blood mononuclear cells isolated from whole blood were treated with BD Cytofix/Cytoperm™ kit for intracellular staining. Cells were then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab20181, 11.117µg/ml) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150117) at 1/2000 dilution for 30 min at 4°C.

      Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 0.005μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

      Acquisition of 10,000 total events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter. Gating strategy – lymphocytes.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (29)

    Publishing research using ab20181? Please let us know so that we can cite the reference in this datasheet.

    ab20181 has been referenced in 29 publications.

    • Choi YW  et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh) 8:2002497 (2021). PubMed: 33643790
    • Meng Q  et al. Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway. J Cell Mol Med 25:1867-1883 (2021). PubMed: 33417281
    • Perugino CA  et al. CD4+ and CD8+ cytotoxic T lymphocytes may induce mesenchymal cell apoptosis in IgG4-related disease. J Allergy Clin Immunol 147:368-382 (2021). PubMed: 32485263
    • Marques P  et al. The role of the tumour microenvironment in the angiogenesis of pituitary tumours. Endocrine 70:593-606 (2020). PubMed: 32946040
    • Liang Y  et al. Exosomal microRNA-144 from bone marrow-derived mesenchymal stem cells inhibits the progression of non-small cell lung cancer by targeting CCNE1 and CCNE2. Stem Cell Res Ther 11:87 (2020). PubMed: 32102682
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-9 of 9 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-HLA-DR antibody [TAL 1B5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Skin)
    Permeabilization
    Yes - 0.5% Triton TBS Ca/Azide
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    MR. Tyler Crowe

    Verified customer

    Submitted Jan 18 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA-DR antibody [TAL 1B5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Appendix)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Ventana CC2 (pH6)
    Permeabilization
    No
    Specification
    Appendix
    Blocking step
    Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 25 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR antibody [TAL 1B5]

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: High pH
    Sample
    Human Tissue sections (Malignant melanoma/lymphocytes)
    Specification
    Malignant melanoma/lymphocytes
    Permeabilization
    No
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 16 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-HLA DR antibody [TAL 1B5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 01 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA DR antibody [TAL 1B5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Monkey Tissue sections (Japanese macaque brain)
    Specification
    Japanese macaque brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer , pressure cooked for 24 minutes
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 20 2012

    Western blot abreview for Anti-HLA DR antibody [TAL 1B5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (human colon lysate)
    Loading amount
    50 µg
    Specification
    human colon lysate
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 09 2012

    Question

    Dear Sir/Mam, I am planning to order the following antibodies however, I could not find satisfactory review of these. I was wondering if you could send me little test samples so that I could make sure that they are working. AB20181 HLADR AB36998 CD204 AB64693 CD206 AB17051 CD163 I hope you will help me with this. Best

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2012

    Answer

    Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

    If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information or see the link below:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 26 2012

    Question

    I found Your suggested HLA-DP/DQ/DR antibody working nicely but in really higher concentration. The other ones like HLA-DQ and HLA-DP are not working. Same with CD74 (ab9514) that we talked about. I tried Your suggestion with room temperature incubation and no signal. I think we should contact and talk about it. You can reach me from 10 to 16 at my office tomorrow.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 15 2011

    Answer

    Here is a link to the fractionation protocol as discussed: https://www.abcam.com/index.html?pageconfig=resource&rid=11473 I really think that enriching for the membrane fraction may help. I will also discuss with one of my colleagues.

    Read More

    Abcam Scientific Support

    Answered on Nov 15 2011

    Question

    Problem with WB with HLA abs

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2011

    Answer

    I am sorry for the delay in responding to you. I tried to call you yesterday and today but was unable to reach you, therefore I would prefer to send an email to ensure a speedy resolution. It is very disappointing that 4 products are not working including the positive control that was provided. It is clear that these 3 antibodies should be working in your samples, especially as another product you purchased form us (ab20181 Anti-HLA DR antibody [TAL 1B5]) is working well. I have just reviewed the datasheet information for ab76562 and there is a mistake on our datasheet as this product has only been tested on recombinant protein therefore we havent tested on human protein. Again I am very sorry for this mistake and have updated the datasheet immediately. I would like to offer 3 replacement antibodies and I have offered some product suggestions below. The images and references should help with product review. However if this is not to your satisfaction I would happily arrange a credit/refund. HLA DR DP DQ ab7856 https://www.abcam.com/HLA-DR--DP--DQ-antibody-CR343-ab7856.html ab8085 https://www.abcam.com/HLA-DR--HLA-DP-antibody-HL-40-ab8085.html ab8094 https://www.abcam.com/HLA-DR--HLA-DP-antibody-MEM-136-ab8094.html ab766 https://www.abcam.com/HLA-DR--HLA-DP-antibody-HL-38-ab766.html HLA DP ab89712 https://www.abcam.com/HLA-DP-antibody-ab89712.html HLA DQA We do not have an antibody tested in human and western blot, although it may be suitable. This product has been tested in flow cytometry only. ab91303 https://www.abcam.com/HLA-DQA1-antibody-HI118-ab91303.html I look forward to your reply with details on how to proceed.

    Read More

    Abcam Scientific Support

    Answered on Sep 23 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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