Anti-HLA DR + DP + DQ antibody [WR18] (Phycoerythrin) (ab23901)

Overview

  • Product name

    Anti-HLA DR + DP + DQ antibody [WR18] (Phycoerythrin)
    See all HLA DR + DP + DQ primary antibodies
  • Description

    Mouse monoclonal [WR18] to HLA DR + DP + DQ (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab23901 recognises the common b unit of HLA-Class 2 molecules. Stains B cells, monocytes and activated T cells.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    B-CLL cells (Human).

  • General notes

    Purified IgG conjugated to R. Phycoerythrin (RPE).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    WR18
  • Myeloma

    NS1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

(or 100ul whole blood).

 

 

 

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD antibody
    • CELIAC 1 antibody
    • CELIAC1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DQ A1 antibody
    • DRB1 antibody
    • DRB4 antibody
    • FLJ27088 antibody
    • FLJ27328 antibody
    • GSE antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR 1 beta antibody
    • HLA class II histocompatibility antigen DR alpha antibody
    • HLA class II histocompatibility antigen DR alpha chain antibody
    • HLA DP histocompatibility type beta 1 subunit antibody
    • HLA DP1B antibody
    • HLA DPB1 antibody
    • HLA DQA antibody
    • HLA DQA1 antibody
    • HLA DQB antibody
    • HLA DQB1 antibody
    • HLA DR1B antibody
    • HLA DR3B antibody
    • HLA DR4B antibody
    • HLA DRA antibody
    • HLA DRA1 antibody
    • HLA DRB1 antibody
    • HLA DRB3 antibody
    • HLA DRB4 antibody
    • HLA DRB5 antibody
    • HLA-DPB1 antibody
    • IDDM 1 antibody
    • IDDM1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex class II DQ alpha 1 antibody
    • Major histocompatibility complex class II DQ beta 1 antibody
    • Major histocompatibility complex class II DR alpha antibody
    • Major histocompatibility complex class II DR beta 1 antibody
    • Major histocompatibility complex class II DR beta 3 antibody
    • Major histocompatibility complex class II DR beta 4 antibody
    • Major histocompatibility complex class II DR beta 5 antibody
    • MGC117330 antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II antigen DRA antibody
    • MHC class II HLA DQ alpha 1 antibody
    • MHC class II HLA DR beta 1 antibody
    • MHC class II HLA DR beta 3 antibody
    • MHC DPB1 antibody
    • MHC DQ beta antibody
    • MHC HLA DPB1 antibody
    • MHC HLA DQ alpha antibody
    see all

References

This product has been referenced in:

  • Brentville VA  et al. T cell repertoire to citrullinated self-peptides in healthy humans is not confined to the HLA-DR SE alleles; Targeting of citrullinated self-peptides presented by HLA-DP4 for tumour therapy. Oncoimmunology 8:e1576490 (2019). Read more (PubMed: 31069134) »
  • Brignone C  et al. A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells. J Immunol 179:4202-11 (2007). Flow Cyt ; Human . Read more (PubMed: 17785860) »
See all 2 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for your enquiry. Unfortunately, there's no concentration available for this conjugate antibody. The antibody's originator recommends using the antibody neat for application in FACS. Please contact us again if you have any additional questions.

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