Anti-HLA DR + HLA DP antibody [MEM-136] (Phycoerythrin) (ab77134)

Overview

  • Product name

    Anti-HLA DR + HLA DP antibody [MEM-136] (Phycoerythrin)
    See all HLA DR + HLA DP primary antibodies
  • Description

    Mouse monoclonal [MEM-136] to HLA DR + HLA DP (Phycoerythrin)
  • Host species

    Mouse
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab77134 recognizes a common epitope on the beta chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta subunit.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human); PHA-activated peripheral blood lymphocytes.

  • Positive control

    • human blood cells
  • General notes

    Conjugates are made from highly purified antibodies (>95% by SDS-PAGE).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: 0.2% BSA, 0.2% PBS

    0.2% BSA (high-grade protease free)
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    The protein A/G purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is then purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
  • Clonality

    Monoclonal
  • Clone number

    MEM-136
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use Neat: 20µl for 100µl whole blood or 106 cells in suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.
    • Cellular localization

      Cell Membrane; Single pass type I membrane protein
    • Database links

    • Alternative names

      • HLA DP antibody
      • HLA DR antibody
      • MHC Class II antibody

    References

    ab77134 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab77134.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up