Overview

  • Product name

    Anti-HLA DRB5 antibody
  • Description

    Rabbit polyclonal to HLA DRB5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HLA DRB5 (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q30154

  • Positive control

    • WB: Raji whole cell and membrane extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab226777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.

Target

  • Function

    Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similarities

    Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Ubiquitinated by MARCH1 and MARCH8 at Lys-254 leading to down-regulation of MHC class II.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. Late endosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Database links

  • Alternative names

    • DR 9 antibody
    • DR beta 5 antibody
    • DR beta 5 chain antibody
    • DR beta-5 antibody
    • DR beta5 antibody
    • DR2 beta 2 antibody
    • DR2-beta-2 antibody
    • DR9 antibody
    • DRB5_HUMAN antibody
    • Dw2 antibody
    • FLJ76359 antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DR beta 5 chain antibody
    • HLA class II histocompatibility antigen DRB1-9 beta chain antibody
    • HLA class II histocompatibility antigen, DR-5 beta chain antibody
    • HLA DRB antibody
    • HLA-DRB5 antibody
    • Human leucocyte antigen DRB5 antibody
    • Leukocyte antigen class II antibody
    • Major histocompatibility complex, class II, DR beta 51 antibody
    • MHC class II antigen DRB1*9 antibody
    • MHC class II antigen DRB5 antibody
    • MHC class II HLA beta chain antibody
    • MHC HLA-DR-beta cell surface glycoprotein antibody
    • MHC HLA-DR-beta chain antibody
    see all

Images

  • All lanes : Anti-HLA DRB5 antibody (ab226777) at 1/5000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) membrane extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS-PAGE gel.

References

ab226777 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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