Overview

  • Product name
    Anti-HLA E antibody [MEM-E/07]
    See all HLA E primary antibodies
  • Description
    Mouse monoclonal [MEM-E/07] to HLA E
  • Host species
    Mouse
  • Specificity
    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified from hybridoma culture supernatant by protein A-affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    MEM-E/07
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 20713594
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

Target

  • Relevance
    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
  • Cellular localization
    Membrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • HLA class I histocompatibility antigen E alpha chain antibody
    • EA1.2 antibody
    • EA2.1 antibody
    • HLA 6.2 antibody
    • HLA class I histocompatibility antigen alpha chain E antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA class I histocompatibility antigen E alpha chain precursor antibody
    • HLA6.2 antibody
    • HLAE antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I E antibody
    • MHC antibody
    • MHC class I antigen E antibody
    • MHC HLA E alpha 1 antibody
    • MHC HLA E alpha 2.1 antibody
    • QA1 antibody
    see all

Images

  • ab11820 staining HLA E in Human endothelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 5% BSA in PBS with 0.1% Triton X-100) for 1 hour at 4°C. An undiluted FITC-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.

    See Abreview

  • Cells were treated with 500 Units of human IFN-gamma on 1 million ECV304 cells after 36 hours cells were harvested (PVG 0.1% trypsin), fixed with 2% paraformaldehyde and surface staining of HLA E was performed using ab11820 at a 1/100 dilution. The secondary used was a goat anti-mouse-FITC, at a 1/100 dilution.
    Red line indicates the untreated expression level and black indicate the induced surface expression of HLA E. Gating strategy - total population.

    See Abreview

  • HLA-G– or HLA-E–expressing HeLa cells were transfected with HCMV endoplasmic reticulum resident transmembrane glycoprotein US10. The lysates were immunoblotted with antibodies against HLA-G, HLA-E (ab11820), or beta-actin as a loading control. The figure shows that expression of HLA-G is slightly reduced by US10.

References

This product has been referenced in:
  • Shwetank  et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). Read more (PubMed: 25086398) »
  • Shwetank  et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . Read more (PubMed: 24236107) »
See all 6 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample
Human Cell (Human endothelial cell line)
Specification
Human endothelial cell line
Permeabilization
Yes - 0.1% triton-x 100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 24 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial)
Loading amount
100 µg
Specification
endothelial
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Human Endothelial cell line (ECV304))
Specification
Human Endothelial cell line (ECV304)
Preparation
Cell harvesting/tissue preparation method: Trypsinisation
Sample buffer: PVG containing 0.1% trypsin
Fixation
2% Paraformaldehyde in PBS
Permeabilization
No
Gating Strategy
total population

Mr. Shwetank Verma

Verified customer

Submitted Mar 16 2011

Answer

I am pleased to be able to provide you with two references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456 If you would like the pdf of the Palmisano paper I can send it to you. I hope this will help you and wish you all the best with your paper. Could you please let us know when you have published it so we can add your reference to the antibody's datasheet, and/or consider submitting an Abreview with your experience of the antibody (we reward Abreviews with points which you can use for future discounts and gifts)?

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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