Key features and details
- Mouse monoclonal [MEM-E/07] to HLA E
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HLA E antibody [MEM-E/07]
See all HLA E primary antibodies
DescriptionMouse monoclonal [MEM-E/07] to HLA E
SpecificityThe antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from hybridoma culture supernatant by protein A-affinity chromatography.
Our Abpromise guarantee covers the use of ab11820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
RelevanceHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
Cellular localizationMembrane; Single-pass type I membrane protein
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
ab11820 staining HLA E in Human endothelial cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/100 in 5% BSA in PBS with 0.1% Triton X-100) for 1 hour at 4°C. An undiluted FITC-conjugated Goat anti-mouse polyclonal was used as the secondary antibody.
Cells were treated with 500 Units of human IFN-gamma on 1 million ECV304 cells after 36 hours cells were harvested (PVG 0.1% trypsin), fixed with 2% paraformaldehyde and surface staining of HLA E was performed using ab11820 at a 1/100 dilution. The secondary used was a goat anti-mouse-FITC, at a 1/100 dilution.
Red line indicates the untreated expression level and black indicate the induced surface expression of HLA E. Gating strategy - total population.
ab11820 has been referenced in 6 publications.
- Shwetank et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). PubMed: 25086398
- Shwetank et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . PubMed: 24236107
- Jagessar SA et al. Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG34-56 peptide in incomplete adjuvant. Eur J Immunol 42:217-27 (2012). PubMed: 21928277
- Jørgensen PB et al. Epstein-Barr virus Peptide Presented by HLA-E is Predominantly Recognized by CD8(bright) Cells in multiple Sclerosis Patients. PLoS One 7:e46120 (2012). Flow Cyt . PubMed: 23049954
- Park B et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . PubMed: 20713594
- Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456