Key features and details
- Mouse monoclonal [MEM-E/07] to HLA E
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HLA E antibody [MEM-E/07]
See all HLA E primary antibodies
DescriptionMouse monoclonal [MEM-E/07] to HLA E
SpecificityThe antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules: HLA B7 (strongly), HLA B8 (moderately), HLA B27, HLA-B44 (weakly).
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to HLA E. The epitope is extracellular. The antibody reacts only with native epitope, not denatured.
Database link: P13747
- IHC-P: Human spleen tissue.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from hybridoma culture supernatant by protein A-affinity chromatography.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 10 µg/ml.
RelevanceHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
Cellular localizationMembrane; Single-pass type I membrane protein
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
ab11820 has been referenced in 7 publications.
- Sasaki T et al. Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8(+) T-cell recognition of stressed cells. Eur J Immunol 46:929-40 (2016). PubMed: 26711740
- Shwetank et al. Inhibition of ERK and proliferation in NK cell lines by soluble HLA-E released from Japanese encephalitis virus infected cells. Immunol Lett 162:94-100 (2014). PubMed: 25086398
- Shwetank et al. Infection of human endothelial cells by Japanese encephalitis virus: increased expression and release of soluble HLA-E. PLoS One 8:e79197 (2013). Human . PubMed: 24236107
- Jagessar SA et al. Unravelling the T-cell-mediated autoimmune attack on CNS myelin in a new primate EAE model induced with MOG34-56 peptide in incomplete adjuvant. Eur J Immunol 42:217-27 (2012). PubMed: 21928277
- Jørgensen PB et al. Epstein-Barr virus Peptide Presented by HLA-E is Predominantly Recognized by CD8(bright) Cells in multiple Sclerosis Patients. PLoS One 7:e46120 (2012). Flow Cyt . PubMed: 23049954
- Park B et al. The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation. J Exp Med 207:2033-41 (2010). WB ; Human . PubMed: 20713594
- Palmisano GL et al. HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol 66:1-12 (2005). PubMed: 15620456