Product nameAnti-HLA E antibody [MEM-E/08]
See all HLA E primary antibodies
DescriptionMouse monoclonal [MEM-E/08] to HLA E
SpecificityThe antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7.
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from hybridoma culture supernatant by protein A-affinity chromatography.
Our Abpromise guarantee covers the use of ab11821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
|Flow Cyt||Use a concentration of 4 µg/ml.
Tested on HLA-E transfected human cell line LCL 721.221
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
RelevanceHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
Cellular localizationMembrane; Single-pass type I membrane protein
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
ab11821 staining HLA E in Human K562 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour at 24°C. Ab6785(1/100) was used as secondary antibody.
Gating Strategy: Isotrope control
Anti-HLA E antibody [MEM-E/08] (ab11821) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 50 kDa, 68 kDa, 73 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
This product has been referenced in:
- Wang F et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat Immunol 17:514-522 (2016). WB, IP ; Mouse . Read more (PubMed: 27043414) »
- Boura JS et al. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev 2014:N/A (2014). Flow Cyt ; Human . Read more (PubMed: 25279386) »