Anti-HLA E antibody [MEM-E/08] (ab11821)

Reviews (1)Q&A (6)References (7)

Key features and details

  • Mouse monoclonal [MEM-E/08] to HLA E
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HLA E antibody [MEM-E/08]
    See all HLA E primary antibodies

  • Description

    Mouse monoclonal [MEM-E/08] to HLA E

  • Host species

    Mouse

  • Specificity

    The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7.

  • Tested applications

    Suitable for: IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human HLA E.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified from hybridoma culture supernatant by protein A-affinity chromatography.

  • Clonality

    Monoclonal

  • Clone number

    MEM-E/08

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

Tested on HLA-E transfected human cell line LCL 721.221 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

Tested on HLA-E transfected human cell line LCL 721.221 ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.

Target

  • Relevance

    HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

  • Cellular localization

    Membrane; Single-pass type I membrane protein

  • Database links

    • Alternative names

      • HLA class I histocompatibility antigen E alpha chain antibody
      • EA1.2 antibody
      • EA2.1 antibody
      • HLA 6.2 antibody
      • HLA class I histocompatibility antigen alpha chain E antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA class I histocompatibility antigen E alpha chain precursor antibody
      • HLA6.2 antibody
      • HLAE antibody
      • Lymphocyte antigen antibody
      • Major histocompatibility complex class I E antibody
      • MHC antibody
      • MHC class I antigen E antibody
      • MHC HLA E alpha 1 antibody
      • MHC HLA E alpha 2.1 antibody
      • QA1 antibody
      see all

    References (7)

    Publishing research using ab11821? Please let us know so that we can cite the reference in this datasheet.

    ab11821 has been referenced in 7 publications.

    • Wang F  et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat Immunol 17:514-522 (2016). WB, IP ; Mouse . PubMed: 27043414
    • Boura JS  et al. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev 2014:N/A (2014). Flow Cyt ; Human . PubMed: 25279386
    • Soland MA  et al. Modulation of Human Mesenchymal Stem Cell Immunogenicity through Forced Expression of Human Cytomegalovirus US Proteins. PLoS One 7:e36163 (2012). Flow Cyt ; Human . PubMed: 22666319
    • Heidenreich S  et al. Impact of the NK cell receptor LIR-1 (ILT-2/CD85j/LILRB1) on cytotoxicity against multiple myeloma. Clin Dev Immunol 2012:652130 (2012). Flow Cyt ; Human . PubMed: 22844324
    • Kalkunte SS  et al. Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal-fetal interface. J Immunol 182:4085-92 (2009). Flow Cyt ; Human . PubMed: 19299706
    • Tennakoon DK  et al. Therapeutic induction of regulatory, cytotoxic CD8+ T cells in multiple sclerosis. J Immunol 176:7119-29 (2006). Flow Cyt ; Human . PubMed: 16709875
    • Brooks CR  et al. The inhibitory receptor NKG2A determines lysis of vaccinia virus-infected autologous targets by NK cells. J Immunol 176:1141-7 (2006). Flow Cyt ; Human . PubMed: 16434388

    Customer reviews and Q&As

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    1-7 of 7 Abreviews or Q&A

    Flow Cytometry abreview for Anti-HLA E antibody [MEM-E/08]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (K562)
    Specification
    K562
    Fixation
    NONE
    Permeabilization
    No
    Gating Strategy
    iostype control
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 20 2010

    Question

    In the data sheet for the HLA-E (MEM-E/08) antibody following crossreactivity is mentioned: The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7 Unfortunatly there are no literature references provides to get informed how much cross reactivity is effecting results. Could you send me the literature references?

    Read More
    Answer

    Thank you for your patience in this matter. The originator of ab11821 (HLA E antibody [MEM-E/08]) has provided the following references: Sibilio L. et al., Tissue Antigens 62, 339, 2003. (Abstract 45 from the 3rd Int. Conference on HLA-G, Paris, July 2003). Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pist illo MP.: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66(1):1-12. I hope this helps.

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    Question

    I am trying to titrate the antibody but need the appropriate cells/conditions, because HLA-E is not constitutively expressed on the surface of all cells. I tried fibroblasts and saw some slight internal staining, but only at the 1:12.5 dilution, which is very weak. Once I establish the correct titer to use, I can test the antibody for cross reactivity with rhesus macaque cells, the ultimate goal. How was this antibody tested for application in FACS? What dilution and samples were used?

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    Answer

    This antibody was tested on the human cell line LCL 721.221 (HLA-E transfected), dilution 1:50 - 1:500. This antibody reacts with the native molecule of HLA-E. I hope this helps. Please contact us again if you have any additional questions.

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    Question

    It is believed that HLA-E is universal expressed on lymphocyte (Human Immunology 1990 (29),131 & Tissue Antigen 1999 (53),292). I used primary aortic endothelial cells and peripheral lymphocytes to test this antibody. Both of them showed negative results. I don't know it is because the expression level is too low to see or other reasons. Did you test this antibody on lymphocyte?

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    Answer

    Thank you for your enquiry. This antibody perfectly works in flow cytometry but as far as we know, this antibody was not tested on lymphocytes. It was tested in FACS with HLA-E transfected human cell line LCL 721.221. Have you tried titrating the antibody? We generally recommend a starting point of 1ug/ml per 1x10^6 cells. However, since we don't have a recommended dilution on the datasheet, it would be important to optimize. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    What types of cells has this antibody been tested on in FACS?

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    Answer

    Thank you for your equiry. For testing this antibody on FACS was used human cell line LCL 721.221, HLA-E transfected. Please do not hesitate to contact us if you need anything further.

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    Question

    I wish to use the above antibody for Flow cytometry on HeLa lines, but am unsure at what levels HLA-E is expressed on these cells. Do you have any data on the level of HLA-E expression demonstrated by HeLa cells?

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    Answer

    Thank you for your enquiry and your interest in our products. This antibody perfectly works in flow cytometry but we have not used HeLa cell line for this study. Perhaps this information can be found in some published paper.

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    Question

    I was interested in purchasing MEM-E/08 for flow cytometry. could you confirm whether or not this reagen interacts with HLa-Cw*0401 and send me the reference for the specificty testing of this antibody.

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    Answer

    The antibody appears to be very specific and most likely will not reacts with the allele of question. We will update our on-line datasheet with the cross-reactivity results very soon.

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