Product nameHLA E overexpression 293T lysate (whole cell)
DescriptionHLA E overexpression lysate
ab94202 is a 293T cell transfected lysate in which Human HLA E has been transiently over-expressed using a pCMV-HLA E plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
Concentration information loading...
BackgroundHLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.
Our Abpromise guarantee covers the use of ab94202 in the following tested applications.
|WB||Use at an assay dependent dilution.|
ab94202 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-HLA E antibody (ab88090) at 1/500 dilution
Lane 1 :
HLA E overexpression 293T lysate (whole cell) (ab94202)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94202 has not yet been referenced specifically in any publications.