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Immunology Adaptive Immunity MHC Class I
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RecombinantRabMAb

Recombinant Anti-HLA F antibody [EPR6803] (ab126624)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)
  • Western blot - Anti-HLA F antibody [EPR6803] (ab126624)
  • OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624)
  • Anti-HLA F antibody [EPR6803] (ab126624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6803] to HLA F
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-HLA F antibody [EPR6803]
    See all HLA F primary antibodies
  • Description

    Rabbit monoclonal [EPR6803] to HLA F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human HLA F (P30511).

  • Positive control

    • Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.52 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6803
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I

Associated products

  • Alternative Versions

    • Anti-HLA F antibody [EPR6803] - BSA and Azide free (ab248133)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human HLA F protein (ab181899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
IHC-P (1)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Involved in the presentation of foreign antigens to the immune system.
    • Sequence similarities

      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P30511 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3134 Human
      • Omim: 143110 Human
      • SwissProt: P30511 Human
      • Unigene: 519972 Human
      • Alternative names

        • CDA12 antibody
        • HLA 5.4 antibody
        • HLA class I histocompatibility antigen, alpha chain F antibody
        • HLA class I molecule antibody
        • HLA F antigen antibody
        • HLA-CDA12 antibody
        • HLA-F antibody
        • HLAF antibody
        • HLAF_HUMAN antibody
        • Leukocyte antigen F antibody
        • MHC class I antigen F antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)

        ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-HLA F antibody [EPR6803] (ab126624)
        Western blot - Anti-HLA F antibody [EPR6803] (ab126624)
        All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution

        Lane 1 : HT1376 cell lysate
        Lane 2 : Human tonsil lysate
        Lane 3 : JAR cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 39 kDa

      • OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624)
        OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-HLA F antibody [EPR6803] (ab126624)
        Anti-HLA F antibody [EPR6803] (ab126624)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab126624? Please let us know so that we can cite the reference in this datasheet.

      ab126624 has been referenced in 1 publication.

      • Wu B  et al. High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma. Anal Cell Pathol (Amst) 2018:7691704 (2018). PubMed: 30510890

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA F antibody [EPR6803]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Placenta)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
      Permeabilization
      Yes - Wash Buffer from Dako with Tween
      Specification
      Placenta
      Fixative
      Paraformaldehyde
      Read More

      Mr. Rudolf Jung

      Verified customer

      Submitted Feb 19 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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