- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-HLA F antibody [EPR6803]
See all HLA F primary antibodies -
DescriptionRabbit monoclonal [EPR6803] to HLA F
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to residues in Human HLA F (P30511).
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Positive control
- Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 2.52 x 10 -11 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR6803
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab126624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa). | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionInvolved in the presentation of foreign antigens to the immune system.
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Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 3134 Human
- Omim: 143110 Human
- SwissProt: P30511 Human
- Unigene: 519972 Human
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Alternative names
- CDA12 antibody
- HLA 5.4 antibody
- HLA class I histocompatibility antigen, alpha chain F antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/HLA-F-Primary-antibodies-ab126624-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry. -
![Western blot - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/HLA-F-Primary-antibodies-ab126624-1.jpg)
Western blot - Anti-HLA F antibody [EPR6803] (ab126624)All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution
Lane 1 : HT1376 cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa -
![Other - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/HLA-F-Primary-antibodies-ab126624-4.jpg)
Other - Anti-HLA F antibody [EPR6803] (ab126624)
References
ab126624 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"