Recombinant Anti-HLA F antibody [EPR6803] (ab126624)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/ab126624-140621-anti-hla-f-antibody-epr6803-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6803] to HLA F
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-HLA F antibody [EPR6803]
See all HLA F primary antibodies -
Description
Rabbit monoclonal [EPR6803] to HLA F -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to residues in Human HLA F (P30511).
-
Positive control
- Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
-
General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.52 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6803 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
|
|
| IHC-P | (1) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Involved in the presentation of foreign antigens to the immune system. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3134 Human
- Omim: 143110 Human
- SwissProt: P30511 Human
- Unigene: 519972 Human
-
Alternative names
- CDA12 antibody
- HLA 5.4 antibody
- HLA class I histocompatibility antigen, alpha chain F antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)
ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/ab126624-140620-anti-hla-f-antibody-epr6803-western-blot.jpg)
Western blot - Anti-HLA F antibody [EPR6803] (ab126624)All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution
Lane 1 : HT1376 cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa -
![OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/ab126624-140619-anti-hla-f-antibody-epr6803.jpg)
OI-RD Scanning - Anti-HLA F antibody [EPR6803] (ab126624) -
![Anti-HLA F antibody [EPR6803] (ab126624)](https://www.abcam.com/ps/products/126/ab126624/Images/ab126624-4-benefits-of-recombinant-antibodies.png)
Anti-HLA F antibody [EPR6803] (ab126624)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab126624 has been referenced in 1 publication.
- Wu B et al. High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma. Anal Cell Pathol (Amst) 2018:7691704 (2018). PubMed: 30510890