Recombinant
RabMAb

Recombinant Anti-HLA F antibody [EPR6803] (ab126624)

Reviews (1) Submit a question

Overview

  • Product name

    Anti-HLA F antibody [EPR6803]
    See all HLA F primary antibodies

  • Description

    Rabbit monoclonal [EPR6803] to HLA F

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human HLA F (P30511).

  • Positive control

    • Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.52 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6803

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Involved in the presentation of foreign antigens to the immune system.

    • Sequence similarities

      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CDA12 antibody
        • HLA 5.4 antibody
        • HLA class I histocompatibility antigen, alpha chain F antibody
        • HLA class I molecule antibody
        • HLA F antigen antibody
        • HLA-CDA12 antibody
        • HLA-F antibody
        • HLAF antibody
        • HLAF_HUMAN antibody
        • Leukocyte antigen F antibody
        • MHC class I antigen F antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA F antibody [EPR6803] (ab126624)

        ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-HLA F antibody [EPR6803] (ab126624)
        Western blot - Anti-HLA F antibody [EPR6803] (ab126624)
        All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution

        Lane 1 : HT1376 cell lysate
        Lane 2 : Human tonsil lysate
        Lane 3 : JAR cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 39 kDa

      • Other - Anti-HLA F antibody [EPR6803] (ab126624)
        Other - Anti-HLA F antibody [EPR6803] (ab126624)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab126624 has not yet been referenced specifically in any publications.

      Publishing research using ab126624? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HLA F antibody [EPR6803]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Placenta)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
      Permeabilization
      Yes - Wash Buffer from Dako with Tween
      Specification
      Placenta
      Fixative
      Paraformaldehyde
      Read More

      Mr. Rudolf Jung

      Verified customer

      Submitted Feb 19 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com