Product nameAnti-HLA F antibody [EPR6803]
See all HLA F primary antibodies
DescriptionRabbit monoclonal [EPR6803] to HLA F
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide, corresponding to residues in Human HLA F (P30511).
- Human tonsil tissue; Human tonsil, HT1376 and JAR cell lysates.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.52 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 39 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the presentation of foreign antigens to the immune system.
Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
- Information by UniProt
- CDA12 antibody
- HLA 5.4 antibody
- HLA class I histocompatibility antigen, alpha chain F antibody
ab126624, at a 1/250 dilution, staining HLA F in paraffin-embedded Human tonsil tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-HLA F antibody [EPR6803] (ab126624) at 1/1000 dilution
Lane 1 : HT1376 cell lysate
Lane 2 : Human tonsil lysate
Lane 3 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab126624 has not yet been referenced specifically in any publications.