Overview

  • Product name
    Anti-HLA G antibody [4H84]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [4H84] to HLA G
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, WB, IP, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HLA G aa 61-83.
    Sequence:

    DSDSACPRMEPRAPWVEQEGPEY

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from hybridoma culture supernatant by protein-A affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    4H84
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity
    Expressed in trophoblasts.
  • Sequence similarities
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • ab52455 staining HLA G in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized Wash Buffer with Tween; antigen retrieval was by heat mediation in a TRIS-EDTA Buffer (pH 9.0). Samples were incubated with primary antibody (1/50 in diluent) for 30 minutes at 20°C. An undiluted Goat anti-Mouse HRP polyclonal was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Xu C  et al. The microRNA-10a/ID3/RUNX2 axis modulates the development of Ossification of Posterior Longitudinal Ligament. Sci Rep 8:9225 (2018). Read more (PubMed: 29907859) »
  • Horii M  et al. Human pluripotent stem cells as a model of trophoblast differentiation in both normal development and disease. Proc Natl Acad Sci U S A 113:E3882-91 (2016). ICC/IF . Read more (PubMed: 27325764) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Human placental explants)
Antigen retrieval step
None
Permeabilization
No
Specification
Human placental explants
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Dr. Angela Crespo

Verified customer

Submitted Sep 15 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester decidua)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization
No
Specification
First trimester decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Ruhul Choudhury

Verified customer

Submitted Jan 24 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (NPC)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NPC
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 09 2017

Question
Answer

Merci de votre appel de toutà l'heure.

Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab33745.


ab52455

gb|AAM90626.1| MHC class I RT1-Au heavy chain precursor [Rattus norvegicus]
Length=157
GENE ID: 24737 RT1-EC2 | RT1 class Ib, locus EC2 [Rattus norvegicus]
(Over 10 PubMed links)
Score = 54.9 bits (122), Expect = 3e-11
Identities = 19/28 (68%), Positives = 19/28 (68%), Gaps = 2/28 (7%)



Query 1 DSDSACPRMEPRAPWVEQEGPEY--RAT 26
DSD PRMEPRA W E EGPEY R T

Sbjct 64 DSDAENPRMEPRARWMEREGPEYWERET 91




ab76869


ref|NP_861544.1| mitochondrial Rho GTPase 2 [Rattus norvegicus]
sp|Q7TSA0.1|MIRO2_RAT RecName: Full=Mitochondrial Rho GTPase 2; Short=MIRO-2; AltName:
Full=Ras homolog gene family member T2
gb|AAP60015.1| http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?direct=on&gbgi=31872401&THE_BLAST_RID=518AP52M01N&log$=mapalign&blast_rank=1MIRO2 precursor [Rattus norvegicus]
Length=622
GENE ID: 287156 Rhot2 | ras homolog gene family, member T2 [Rattus norvegicus]
(10 or fewer PubMed links)
Score = 27.8 bits (58), Expect = 0.018
Identities = 11/15 (73%), Positives = 11/15 (73%), Gaps = 2/15 (13%)

Query 1 VLAAVVTGAAVAAVL 15
VL AV G AVAAVL

Sbjct 598 VLVAV--GTAVAAVL 610



Comme nous n'avons pas d’échantillon gratuit à des fins de tests préliminaires je ne peux pas vous en offrir un. Cependant, si vous souhaitez tester ab52455 chez le rat, il me sera possible de vous offrir une remise (exceptionnellement, car l'alignement n'est pas si bon). Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab[XXX] pourra être utilisable lors d’une prochaine commande d’anticorps primaire.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriez tester ab52455 chez le rat afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab52455
2. Acheter ab52455 par téléphone, fax ou internet (www.abcam.com)
3. Le tester chez le rat
4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab52455 est démontré ne pas fonctionner chez le rat, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.
N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.
Termes et Conditions : www.abcam.com/collaborationdiscount.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Specification
spleen
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
Permeabilization
Yes - DAKO Wash-Buffer with Tween

Mr. Rudolf Jung

Verified customer

Submitted May 01 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with ab7759, order number ******. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (JEG-3)
Specification
JEG-3
Fixation
None
Permeabilization
No
Gating Strategy
iostype control

Abcam user community

Verified customer

Submitted May 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (JEG-3)
Loading amount
30 µg
Specification
JEG-3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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