Anti-HLA G antibody [4H84] (ab52455)
Key features and details
- Mouse monoclonal [4H84] to HLA G
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
-
Product name
Anti-HLA G antibody [4H84]
See all HLA G primary antibodies -
Description
Mouse monoclonal [4H84] to HLA G -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HLA G aa 61-83.
Sequence:DSDSACPRMEPRAPWVEQEGPEY
-
Positive control
- WB: JEG-3 cell line.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4H84 -
Isotype
IgG1 -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
Use a concentration of 10 µg/ml.
|
WB | (2) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa. |
Target
-
Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
-
Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Images
-
All lanes : Anti-HLA G antibody [4H84] (ab52455) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCaP cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCaP cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse
Predicted band size: 38 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Western blotting analysis of human HLA-G using ab52455 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab52455 followed by IRDye800-conjugated anti-mouse secondary antibody.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (32)
ab52455 has been referenced in 32 publications.
- Castel G & David L Induction of human trophoblast stem cells. Nat Protoc 17:2760-2783 (2022). PubMed: 36241723
- Chen H et al. Potential role of FoxO3a in the regulation of trophoblast development and pregnancy complications. J Cell Mol Med 25:4363-4372 (2021). PubMed: 33811439
- Wang M et al. Galectin-14 Promotes Trophoblast Migration and Invasion by Upregulating the Expression of MMP-9 and N-Cadherin. Front Cell Dev Biol 9:645658 (2021). PubMed: 33796532
- Guo G et al. Human naive epiblast cells possess unrestricted lineage potential. Cell Stem Cell 28:1040-1056.e6 (2021). PubMed: 33831366
- Horii M et al. Modeling preeclampsia using human induced pluripotent stem cells. Sci Rep 11:5877 (2021). PubMed: 33723311