Western blot abreview for Anti-HLA G antibody [4H84]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (JEG-3)
Loading amount
30 µg
Specification
JEG-3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C

Secondary antibody

Name
Non-Abcam antibody was used: alkaline phosphatease-conjuagted anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
BCIP/NBT
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 39, 37, 27, 23, 18 kDa Non-specific: 45 kDa

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Submitted May 05 2010

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