Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HLA G antibody [4H84]

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Human placental explants)
Antigen retrieval step
None
Permeabilization
No
Specification
Human placental explants
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 2% FBS
Concentration
20 µg/ml

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 488

Additional data

Additional Notes
Tissue sections of human placental villi explants cultured in Matrigel-coated Transwells for 96 hours. Tissue was fixed with 4% paraformaldehyde, subjected to sucrose gradient for water removal, embedded in OCT medium and flash frozen. 5 ´m sections were cut in a cryostat, adhered to glass slides and allowed to air dry. Slides were washed in PBS, blocked with 10% BSA and stained overnight with 20 ´g/ml HLA-G antibody (clone 4H84, Abcam #ab52455), followed by staining with donkey-anti-mouse AlexFluor 488 secondary antibody. Nuclei were stained with DAP. Scale bars: 50 ´m.

Dr. Angela Crespo

Verified customer

Submitted Sep 15 2017

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