Overview

  • Product name
    Anti-HLA G antibody [4H84] (Biotin)
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [4H84] to HLA G (Biotin)
  • Host species
    Mouse
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HLA G aa 61-83.
    Sequence:

    DSDSACPRMEPRAPWVEQEGPEY


    Database link: P17693

  • Positive control
    • WB: HLA-G1 transfectants (LCL-HLA-G1).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Prior to conjugation ab52456 was purified by Protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    4H84
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • Function
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity
    Expressed in trophoblasts.
  • Sequence similarities
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • Anti-HLA G antibody [4H84] (Biotin) (ab52456) at 2 µg/ml + HLA-G1 transfectants (LCL-HLA-G1)

    Developed using the ECL technique.

    Predicted band size: 38 kDa

References

This product has been referenced in:
  • Moreau P  et al. HLA-G gene repression is reversed by demethylation. Proc Natl Acad Sci U S A 100:1191-6 (2003). WB, ICC ; Human . Read more (PubMed: 12552087) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab52456.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up