Overview

  • Product name

    Anti-HLA G antibody [5A6G7]
    See all HLA G primary antibodies
  • Description

    Mouse monoclonal [5A6G7] to HLA G
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to HLA G. C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 protein coupled to ovalbumin

  • Positive control

    • JEG3 cell lysate; HeLa/ M8 - HLA G5/6 transfected cell lysates, HeLa/HLA-G5 cell supernatant; placenta tissue.
  • General notes


    ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS. Purity >95% by SDS-PAGE.
  • Primary antibody notes

    ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
  • Clonality

    Monoclonal
  • Clone number

    5A6G7
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Can be as capture antibody to analyze of soluble HLA G together with a suitable detection antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 10 - 20 µg/ml.
IHC-Fr Use a concentration of 10 - 20 µg/ml.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity

    Expressed in trophoblasts.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • ab76869 staining HLA G in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-HLA G antibody [5A6G7] (ab76869)

    Lane 1 : M8 cell line transfected with empty vector
    Lane 2 : M8 cell line transfected with HLA G1

    Lane 3 : M8 cell line transfected with HLA G5

    Lane 4 : M8 cell line transfected with HLA G6


    Predicted band size: 38 kDa
    Observed band size: 30,38 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Wan R  et al. Human Leukocyte Antigen-G Inhibits the Anti-Tumor Effect of Natural Killer Cells via Immunoglobulin-Like Transcript 2 in Gastric Cancer. Cell Physiol Biochem 44:1828-1841 (2017). Read more (PubMed: 29224003) »
  • Du MR  et al. Embryonic trophoblasts induce decidual regulatory T cell differentiation and maternal-fetal tolerance through thymic stromal lymphopoietin instructing dendritic cells. J Immunol 192:1502-11 (2014). Flow Cyt ; Human . Read more (PubMed: 24453244) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Placenta
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Mar 09 2016

Question
Answer

Merci de votre appel de toutà l'heure.

Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab33745.


ab52455

gb|AAM90626.1| MHC class I RT1-Au heavy chain precursor [Rattus norvegicus]
Length=157
GENE ID: 24737 RT1-EC2 | RT1 class Ib, locus EC2 [Rattus norvegicus]
(Over 10 PubMed links)
Score = 54.9 bits (122), Expect = 3e-11
Identities = 19/28 (68%), Positives = 19/28 (68%), Gaps = 2/28 (7%)



Query 1 DSDSACPRMEPRAPWVEQEGPEY--RAT 26
DSD PRMEPRA W E EGPEY R T

Sbjct 64 DSDAENPRMEPRARWMEREGPEYWERET 91




ab76869


ref|NP_861544.1| mitochondrial Rho GTPase 2 [Rattus norvegicus]
sp|Q7TSA0.1|MIRO2_RAT RecName: Full=Mitochondrial Rho GTPase 2; Short=MIRO-2; AltName:
Full=Ras homolog gene family member T2
gb|AAP60015.1| http://www.ncbi.nlm.nih.gov/mapview/map_search.cgi?direct=on&gbgi=31872401&THE_BLAST_RID=518AP52M01N&log$=mapalign&blast_rank=1MIRO2 precursor [Rattus norvegicus]
Length=622
GENE ID: 287156 Rhot2 | ras homolog gene family, member T2 [Rattus norvegicus]
(10 or fewer PubMed links)
Score = 27.8 bits (58), Expect = 0.018
Identities = 11/15 (73%), Positives = 11/15 (73%), Gaps = 2/15 (13%)

Query 1 VLAAVVTGAAVAAVL 15
VL AV G AVAAVL

Sbjct 598 VLVAV--GTAVAAVL 610



Comme nous n'avons pas d’échantillon gratuit à des fins de tests préliminaires je ne peux pas vous en offrir un. Cependant, si vous souhaitez tester ab52455 chez le rat, il me sera possible de vous offrir une remise (exceptionnellement, car l'alignement n'est pas si bon). Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab[XXX] pourra être utilisable lors d’une prochaine commande d’anticorps primaire.
Etapes à suivre :
1. Nous confirmer que vous souhaiteriez tester ab52455 chez le rat afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab52455
2. Acheter ab52455 par téléphone, fax ou internet (www.abcam.com)
3. Le tester chez le rat
4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews
5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
Même si ab52455 est démontré ne pas fonctionner chez le rat, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.
N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.
Termes et Conditions : www.abcam.com/collaborationdiscount.

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