Product nameAnti-HLA G antibody [5A6G7]
See all HLA G primary antibodies
DescriptionMouse monoclonal [5A6G7] to HLA G
Tested applicationsSuitable for: ELISA, WB, IHC-P, IHC-Fr, ICC, Flow Cytmore details
Species reactivityReacts with: Human
Other Immunogen Type corresponding to HLA G. C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 protein coupled to ovalbumin
- JEG3 cell lysate; HeLa/ M8 - HLA G5/6 transfected cell lysates, HeLa/HLA-G5 cell supernatant; placenta tissue.
ab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS. Purity >95% by SDS-PAGE.
Primary antibody notesab76869 allows discrimination between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
Our Abpromise guarantee covers the use of ab76869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Can be as capture antibody to analyze of soluble HLA G together with a suitable detection antibody.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.|
|IHC-P||Use a concentration of 10 - 20 µg/ml.|
|IHC-Fr||Use a concentration of 10 - 20 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 2 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
Tissue specificityExpressed in trophoblasts.
Sequence similaritiesBelongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
- Information by UniProt
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
ab76869 staining HLA G in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/50 in blocking buffer) for 30 minutes at 20°C. A HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
All lanes : Anti-HLA G antibody [5A6G7] (ab76869)
Lane 1 : M8 cell line transfected with empty vector
Lane 2 : M8 cell line transfected with HLA G1
Lane 3 : M8 cell line transfected with HLA G5
Lane 4 : M8 cell line transfected with HLA G6
Predicted band size: 38 kDa
Observed band size: 30,38 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Leisegang MS et al. The histone demethylase PHF8 facilitates alternative splicing of the histocompatibility antigen HLA-G. FEBS Lett 593:487-498 (2019). Read more (PubMed: 30758047) »
- Wan R et al. Human Leukocyte Antigen-G Inhibits the Anti-Tumor Effect of Natural Killer Cells via Immunoglobulin-Like Transcript 2 in Gastric Cancer. Cell Physiol Biochem 44:1828-1841 (2017). Read more (PubMed: 29224003) »