Overview

  • Product name

  • Description

    Rabbit polyclonal to HLA G
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 68-98 of Human HLA G.

  • Positive control

    • NCI-H460 cell line lysate; Human lung carcinoma tissue; NCI-H460 cells; 293 cell lysates transiently transfected with the HLA G gene.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135736 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity

    Expressed in trophoblasts.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • Flow cytometric analysis of NCI-H460 cells labelling HLA G with ab135736 at 1/10 dilution (green) compared to negative control cells (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Anti-HLA G antibody (ab135736) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size: 38 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma labelling HLA G with ab135736 with 1/50 dilution, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • All lanes : Anti-HLA G antibody (ab135736) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the HLA G gene.

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 38 kDa

References

ab135736 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab135736.
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