Overview

  • Product name
    Anti-HLA G antibody [MEM-G/1]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/1] to HLA G
  • Host species
    Mouse
  • Specificity
    The antibody reacts with denaturated HLA-G heavy chain.
  • Tested applications
    Suitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human, Macaque monkey
  • Immunogen

    Recombinant full length denatured heavy chain (Human).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Purified from TCS
  • Clonality
    Monoclonal
  • Clone number
    MEM-G/1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 38.2 kDa. Does not react with native HLA-G on cell surface.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity
    Expressed in trophoblasts.
  • Sequence similarities
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
  • All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 38.2 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Swets M  et al. HLA-G protein expression in colorectal cancer evaluated by immunohistochemistry and western blot analysis: Its expression characteristics remain enigmatic. Clin Immunol 194:80-86 (2018). Read more (PubMed: 30006120) »
  • Guo W  et al. Decreased Human Leukocyte Antigen-G Expression by miR-133a Contributes to Impairment of Proinvasion and Proangiogenesis Functions of Decidual NK Cells. Front Immunol 8:741 (2017). WB . Read more (PubMed: 28702027) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Placenta
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Feb 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (JEG-3)
Loading amount
30 µg
Specification
JEG-3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Jun 01 2010

Answer

Thank you for your enquiry. I can confirm that the recombinant protein was produced in bacteria. If there is anything else that I can help you with, please do not hesitate to contact me.

Read More

Question
Answer

Thank you for your enquiry. I would recommend a starting dilution of 1/100, a concentration of 10 ug/mL, for this antibody in IHC-P. It is quite common to use a concentration between 1-20 ug/mL for IHC experiments. For this particular antibody we have received a review from a customer in which the antibody was used at a dilution of 1/200 on formalin fixed sections of human placenta extravillous cytotrophoblasts (not paraffin embedded). My recommendation of 1/100 is a starting dilution and you may have to optimize this dilution for your tissue. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Placenta, extravillous cytotrophoblasts)
Specification
Placenta, extravillous cytotrophoblasts

Mr. Richard Bruggeman

Verified customer

Submitted Jun 30 2005

Answer

Thank you for your enquiry regarding ab7759. The product datasheet for this antibody can be viewed by logging onto our website at www.Abcam.com and entering ab7759 in the search field. All relevant information such as price, technical data and availability can be viewed on this datasheet. To place an order, please get in touch with one of our experienced distribution partner, Gene Research Lab Co Ltd 3Fl., No.32, Sec.2, Jungyang S.Rd Beitou Dist Taipei Taiwan 112 ROC Email:sue@gene-rl.com Phone:+886-2-28925609 Fax:+882-2-28925602 Web:www.gene-rl.com Please contact us again if you require further assistance.

Read More

Answer

Thank you for your enquiry and your interest in our products. We would like to confirm that ab7759 is a monoclonal antibody which recognizes human HLA-G1 and MEM-G/1 is the clone number. Please take a look at the on-line datasheet of this product, you will find useful information.

Read More

Question
Answer

HLA-G1 usually moves as 39 kDa band. Other isoforms (G2, G3, G4, G5,) are also detected if present in your sample.

Read More

Answer

Try this antibody at 1:100 and adjust accordingly from the results obtained. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6.0, 15 min.

Read More

Answer

This antibody reacts with denaturated heavy chain of HLA-G. Routine immunohistochemical protocol for staining of formalin-fixed, paraffin embedded sections is applicable. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6, 15 min.

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up