Overview

  • Product name

    Anti-HLA G antibody [MEM-G/1]
    See all HLA G primary antibodies
  • Description

    Mouse monoclonal [MEM-G/1] to HLA G
  • Host species

    Mouse
  • Specificity

    The antibody reacts with denaturated HLA-G heavy chain.
  • Tested applications

    Suitable for: IHC-P, WB, IHC-FoFrmore details
  • Species reactivity

    Reacts with: Human, Macaque monkey
  • Immunogen

    Recombinant full length denatured heavy chain (Human).

  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-G/1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 38.2 kDa.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity

    Expressed in trophoblasts.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
  • All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 38.2 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Swets M  et al. HLA-G protein expression in colorectal cancer evaluated by immunohistochemistry and western blot analysis: Its expression characteristics remain enigmatic. Clin Immunol 194:80-86 (2018). Read more (PubMed: 30006120) »
  • Guo W  et al. Decreased Human Leukocyte Antigen-G Expression by miR-133a Contributes to Impairment of Proinvasion and Proangiogenesis Functions of Decidual NK Cells. Front Immunol 8:741 (2017). WB . Read more (PubMed: 28702027) »
See all 18 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Placenta
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Feb 18 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (JEG-3)
Loading amount
30 µg
Specification
JEG-3
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Jun 01 2010

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Placenta, extravillous cytotrophoblasts)
Specification
Placenta, extravillous cytotrophoblasts

Mr. Richard Bruggeman

Verified customer

Submitted Jun 30 2005

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