Question (5150) | Anti-HLA G antibody [MEM-G/1] (ab7759)

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Question

I noticed that the antibody does not react with native HLA-G. Therefore, is a special procedure required for immunohistochemistry, specifically formalin fixed sections?

Answer

This antibody reacts with denaturated heavy chain of HLA-G. Routine immunohistochemical protocol for staining of formalin-fixed, paraffin embedded sections is applicable. Recommended epitope retrieval is performed by microwave heating in citrate buffer, pH 6, 15 min.

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