Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-HLA G antibody [MEM-G/1]

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Placenta, extravillous cytotrophoblasts)
Specification
Placenta, extravillous cytotrophoblasts

Other product details

Dilution
1/200
Incubation time
0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: DakoCytomation Envision+ Anti-Mouse/HRP (RTU)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Although we have yet to use this antibody on our batch of experimental tissue, the results that we obtained from our titration runs were very promising. The staining appeared to be very specific and robust, with very little to no non-specific background staining.


Staining Summary & Notes:

1mM EDTA Buffer, pH 8.0, HIER for 20 minutes in steamer
Rinse with TBS containing 0.05% Tween 20
10 minute blocking in 3% Peroxide
Rinse with TBS containing 0.05% Tween 20
30 minutes in Primary Antibody @ RT
Rinse with TBS containing 0.05% Tween 20
30 minutes in Secondary Antibody @ RT
Rinse with TBS containing 0.05% Tween 20
10 minutes in DAB+ (DakoCytomation) @ RT
Water Rinse
Counterstain for 6 minutes in Mayer's Hematoxylin (DakoCytomation) @ RT

Mr. Richard Bruggeman

Verified customer

Submitted Jun 30 2005

Sign up