Overview

  • Product name
    Anti-HLA G antibody [MEM-G/11]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/11] to HLA G
  • Host species
    Mouse
  • Specificity
    HLA G antigen.
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) refolded with beta 2 microglobulin and peptide.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    MEM-G/11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 17549736
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

The antibody MEM-G/11 does not cross-block standard MEM-G/9 antibody.

 

 

 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity
    Expressed in trophoblasts.
  • Sequence similarities
    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

References

This product has been referenced in:
  • Apps R  et al. A homodimeric complex of HLA-G on normal trophoblast cells modulates antigen-presenting cells via LILRB1. Eur J Immunol 37:1924-37 (2007). WB . Read more (PubMed: 17549736) »
  • Menier C  et al. Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol 64:315-26 (2003). Read more (PubMed: 12590976) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer




I don't expect any problems with the ab4570 as this one works fine in IP as well, however, I am not sure about the ab42454.

Nonetheless, I am not too concerned either: ab42454 is not tested in IP, but it works in ELISA, which is a good indicator that it will react with the native protein.

Read More

Answer

Antibodies MEM-G/9 and MEM-G/11 recognize HLA-G1 (full length) but not truncated isoforms (G2, G3, G4) on the cell surface. Both react with HLA-G5 (G6 was not rigorously tested), i.e. the soluble isoform. MEM-G/11 reacts with a different epitope than MEM-G/9, this could be useful in double staining (MEM-G/11 is available as FITC and MEM-G/9 as PE conjugate) of cell surface HLA-G1. In a soluble form (actively secreted G5 or shedded G1), it was not demonstrated that both mAbs can be used as pair in sandwich ELISA.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up