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Immunology Adaptive Immunity MHC Class I
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Anti-HLA G antibody [MEM-G/9] (ab7758)

  • Datasheet
  • SDS
Reviews (3)Q&A (3)References (22)

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Key features and details

  • Mouse monoclonal [MEM-G/9] to HLA G
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-HLA G antibody [MEM-G/9]
    See all HLA G primary antibodies
  • Description

    Mouse monoclonal [MEM-G/9] to HLA G
  • Host species

    Mouse
  • Specificity

    Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human HLA G.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    MEM-G/9
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • MHC
    • Class I
    • Immunology
    • Immune System Diseases
    • Allergens

Associated products

  • Alternative Versions

    • PE Anti-HLA G antibody [MEM-G/9] (ab24384)
    • APC Anti-HLA G antibody [MEM-G/9] (ab40915)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human HLA G protein (His tag) (ab225660)
  • Related Products

    • Anti-HLA-DP antibody [B7/21] (ab20897)

Target

  • Function

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity

    Expressed in trophoblasts.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P17693 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3135 Human
    • Omim: 142871 Human
    • SwissProt: P17693 Human
    • Unigene: 512152 Human
    • Alternative names

      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (22)

    Publishing research using ab7758? Please let us know so that we can cite the reference in this datasheet.

    ab7758 has been referenced in 22 publications.

    • Tang L  et al. Deficiency of DICER reduces the invasion ability of trophoblasts and impairs the pro-angiogenic effect of trophoblast-derived microvesicles. J Cell Mol Med 24:4915-4930 (2020). PubMed: 32198822
    • Ajith A  et al. HLA-G dimer targets Granzyme B pathway to prolong human renal allograft survival. FASEB J 33:5220-5236 (2019). PubMed: 30620626
    • Kolanko E  et al. Increased immunomodulatory capacity of human amniotic cells after activation by pro-inflammatory chemokines. Eur J Pharmacol 859:172545 (2019). PubMed: 31319066
    • Azizian S  et al. Immunological compatibility status of placenta-derived stem cells is mediated by scaffold 3D structure. Artif Cells Nanomed Biotechnol 46:876-884 (2018). PubMed: 29475368
    • Wang C  et al. Elimination of CD4lowHLA-G+ T cells overcomes castration-resistance in prostate cancer therapy. Cell Res 28:1103-1117 (2018). PubMed: 30297869
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Question

    Could you please advise me if your HLA-G (ab7758) anti body is PE or FITC conjugated as the data sheet does not say?
    Thanks very much for your help

    Read More

    Abcam community

    Verified customer

    Asked on Jul 05 2012

    Answer

    Thank you for contacting us.

    The antibody is conjugated with neither Fluorescence dye. If this would be the case we would have mentioned this within the name eg. ab7758 Anti-HLA G antibody [MEM-G/9] (PE) and further in the short description. The images you see on the datasheet are created with the help of secondary antibodies, and the corresponding antibody numbers can be found in the image descriptions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 05 2012

    Western blot abreview for Anti-HLA G antibody [MEM-G/9]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jeg-3 cell line)
    Loading amount
    100 µg
    Specification
    Jeg-3 cell line
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 20 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA G antibody [MEM-G/9]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (JEG-3)
    Specification
    JEG-3
    Fixative
    NONE
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 21 2010

    Flow Cytometry abreview for Anti-HLA G antibody [MEM-G/9]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (JEG-3)
    Specification
    JEG-3
    Fixation
    NONE
    Permeabilization
    No
    Gating Strategy
    isotype control
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 20 2010

    Question

    I was interested in your monoclonal antibody to HLA-G, MEM-G/9 (ab7758). Do you know if it recognizes HLA alleles G*01012 or G*01013? I would appreciate any information you have on this.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2002

    Answer

    Unfortunately, no information on the reactivity with G*01012 or G*01013 alleles is available.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2002

    Question

    Hello, Do you know if this would be useful in identifying ovine trophoblasts? (in culture) Thanks, Robert Chang, MD Children's Hospital, Boston USA

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2001

    Answer

    Probably not as the cross reactivity is restricted to primates.

    Read More

    Abcam Scientific Support

    Answered on Sep 24 2001

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