Anti-HLA G antibody [MEM-G/9] (ab7758)
Key features and details
- Mouse monoclonal [MEM-G/9] to HLA G
- Suitable for: IHC-Fr, Flow Cyt, WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-HLA G antibody [MEM-G/9]
See all HLA G primary antibodies -
Description
Mouse monoclonal [MEM-G/9] to HLA G -
Host species
Mouse -
Specificity
Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: IHC-Fr, Flow Cyt, WB, ICC/IF, ELISAmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
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Immunogen
Recombinant full length protein corresponding to Human HLA G.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-G/9 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
Do not fix cells, see Menier et al. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.
The antibody MEM-G/9 reacts both with cell surface and soluble form of HLA-G, but is not suitable for standard WB as it detects native antigen. |
| ICC/IF | (1) |
Use at an assay dependent concentration.
Do not fix cells, see Menier et al. |
| ELISA |
Use at an assay dependent concentration.
See Abreview. |
| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. Do not fix cells, see Menier et al. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. The antibody MEM-G/9 reacts both with cell surface and soluble form of HLA-G, but is not suitable for standard WB as it detects native antigen. |
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ICC/IF
Use at an assay dependent concentration. Do not fix cells, see Menier et al. |
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ELISA
Use at an assay dependent concentration. See Abreview. |
Target
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Function
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
Tissue specificity
Expressed in trophoblasts. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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Alternative names
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab7758 has been referenced in 22 publications.
- Tang L et al. Deficiency of DICER reduces the invasion ability of trophoblasts and impairs the pro-angiogenic effect of trophoblast-derived microvesicles. J Cell Mol Med 24:4915-4930 (2020). PubMed: 32198822
- Ajith A et al. HLA-G dimer targets Granzyme B pathway to prolong human renal allograft survival. FASEB J 33:5220-5236 (2019). PubMed: 30620626
- Kolanko E et al. Increased immunomodulatory capacity of human amniotic cells after activation by pro-inflammatory chemokines. Eur J Pharmacol 859:172545 (2019). PubMed: 31319066
- Azizian S et al. Immunological compatibility status of placenta-derived stem cells is mediated by scaffold 3D structure. Artif Cells Nanomed Biotechnol 46:876-884 (2018). PubMed: 29475368
- Wang C et al. Elimination of CD4lowHLA-G+ T cells overcomes castration-resistance in prostate cancer therapy. Cell Res 28:1103-1117 (2018). PubMed: 30297869