Anti-HLA G antibody [MEM-G/9] (ab7758)

Reviews (3)Q&A (3)References (13)

Overview

  • Product name
    Anti-HLA G antibody [MEM-G/9]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/9] to HLA G
  • Host species
    Mouse
  • Specificity
    Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, ELISA, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human HLA G.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Clonality
    Monoclonal
  • Clone number
    MEM-G/9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Do not fix cells, see Menier et al.
IHC-Fr Use at an assay dependent concentration.
WB 1/2000. The antibody MEM-G/9 reacts both with cell surface and soluble form of HLA-G, but is not suitable for standard WB as it detects native antigen.
ELISA 1/100 - 1/2500. See Abreview.
Flow Cyt Use at an assay dependent concentration.

Do not fix cells, see Menier et al.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
    • Tissue specificity
      Expressed in trophoblasts.
    • Sequence similarities
      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • B2 microglobulin antibody
        • DADB-15K14.8 antibody
        • HLA 6.0 antibody
        • HLA class I histocompatibility antigen alpha chain G antibody
        • HLA class I histocompatibility antigen, alpha chain G antibody
        • HLA class I molecule antibody
        • HLA G antibody
        • HLA G antigen antibody
        • HLA G histocompatibility antigen class I G antibody
        • HLA G3 antibody
        • HLA-G antibody
        • HLA-G histocompatibility antigen, class I antibody
        • HLA60 antibody
        • HLAG antibody
        • HLAG_HUMAN antibody
        • Major histocompatibility complex class I G antibody
        • MHC class I antigen antibody
        • MHC class I antigen G antibody
        • MHC G antibody
        • T-cell A locus antibody
        • TCA antibody
        see all

      Images

      • Flow Cytometry - Anti-HLA G antibody [MEM-G/9] (ab7758)
        Flow Cytometry - Anti-HLA G antibody [MEM-G/9] (ab7758)This image is courtesy of an anonymous Abreview
        ab7758 staining HLA G in Human JEG-3 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour. Ab6785(1/100) was used as secondary antibody.
        Gating Strategy: Isotrope control

        See Abreview

      • Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [MEM-G/9] (ab7758)
        Immunocytochemistry/ Immunofluorescence - Anti-HLA G antibody [MEM-G/9] (ab7758)This image is courtesy of an anonymous Abreview

        ab7758 staining HLA G in Human JEG-3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were blocked with 10% serum for 45 minutes at 24°C. Samples were incubated with primary antibody (1/50) for 1 hour at 24°C. ab6785 (1/100) was used as the secondary antibody.

        See Abreview

      References

      This product has been referenced in:
      • Guo W  et al. Decreased Human Leukocyte Antigen-G Expression by miR-133a Contributes to Impairment of Proinvasion and Proangiogenesis Functions of Decidual NK Cells. Front Immunol 8:741 (2017). Read more (PubMed: 28702027) »
      • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). Read more (PubMed: 26248480) »
      See all 13 Publications for this product

      Publishing research using ab7758? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-6 of 6 Abreviews or Q&A

      Question

      Could you please advise me if your HLA-G (ab7758) anti body is PE or FITC conjugated as the data sheet does not say?
      Thanks very much for your help

      Read More
      Answer

      Thank you for contacting us.

      The antibody is conjugated with neither Fluorescence dye. If this would be the case we would have mentioned this within the name eg. ab7758 Anti-HLA G antibody [MEM-G/9] (PE) and further in the short description. The images you see on the datasheet are created with the help of secondary antibodies, and the corresponding antibody numbers can be found in the image descriptions.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
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      Western blot abreview for Anti-HLA G antibody [MEM-G/9]

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Jeg-3 cell line)
      Loading amount
      100 µg
      Specification
      Jeg-3 cell line
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 20 2012

      Immunocytochemistry/ Immunofluorescence abreview for Anti-HLA G antibody [MEM-G/9]

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (JEG-3)
      Specification
      JEG-3
      Fixative
      NONE
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 21 2010

      Flow Cytometry abreview for Anti-HLA G antibody [MEM-G/9]

       Excellent
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      abreview image
      Application
      Flow Cytometry
      Sample
      Human Cell (JEG-3)
      Specification
      JEG-3
      Fixation
      NONE
      Permeabilization
      No
      Gating Strategy
      isotype control
      Read More

      Abcam user community

      Verified customer

      Submitted Apr 20 2010

      Question

      I was interested in your monoclonal antibody to HLA-G, MEM-G/9 (ab7758). Do you know if it recognizes HLA alleles G*01012 or G*01013? I would appreciate any information you have on this.

      Read More
      Answer

      Unfortunately, no information on the reactivity with G*01012 or G*01013 alleles is available.

      Read More

      Question

      Hello, Do you know if this would be useful in identifying ovine trophoblasts? (in culture) Thanks, Robert Chang, MD Children's Hospital, Boston USA

      Read More
      Answer

      Probably not as the cross reactivity is restricted to primates.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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