Overview

  • Product name
    Anti-HLA G antibody [MEM-G/9]
    See all HLA G primary antibodies
  • Description
    Mouse monoclonal [MEM-G/9] to HLA G
  • Host species
    Mouse
  • Specificity
    Reacts with the native form of human HLA-G on the cell surface as well as with soluble HLA-G molecule in its beta 2-microglobulin associated form. Specificity was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary DNA. This antibody does not compete with a tested panel of other monoclonal antibodies to HLA-G. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, ELISA, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Recombinant full length protein corresponding to Human HLA G.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Clonality
    Monoclonal
  • Clone number
    MEM-G/9
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Do not fix cells, see Menier et al.

IHC-Fr Use at an assay dependent concentration.
WB 1/2000. The antibody MEM-G/9 reacts both with cell surface and soluble form of HLA-G, but is not suitable for standard WB as it detects native antigen.
ELISA 1/100 - 1/2500. See Abreview.
Flow Cyt Use at an assay dependent concentration.

Do not fix cells, see Menier et al.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
    • Tissue specificity
      Expressed in trophoblasts.
    • Sequence similarities
      Belongs to the MHC class I family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • B2 microglobulin antibody
      • DADB-15K14.8 antibody
      • HLA 6.0 antibody
      • HLA class I histocompatibility antigen alpha chain G antibody
      • HLA class I histocompatibility antigen, alpha chain G antibody
      • HLA class I molecule antibody
      • HLA G antibody
      • HLA G antigen antibody
      • HLA G histocompatibility antigen class I G antibody
      • HLA G3 antibody
      • HLA-G antibody
      • HLA-G histocompatibility antigen, class I antibody
      • HLA60 antibody
      • HLAG antibody
      • HLAG_HUMAN antibody
      • Major histocompatibility complex class I G antibody
      • MHC class I antigen antibody
      • MHC class I antigen G antibody
      • MHC G antibody
      • T-cell A locus antibody
      • TCA antibody
      see all

    Images

    • ab7758 staining HLA G in Human JEG-3 cells by Flow Cytometry. The sample was incubated with the primary antibody, (1/50), for 1 hour. Ab6785(1/100) was used as secondary antibody.
      Gating Strategy: Isotrope control

      See Abreview

    • ab7758 staining HLA G in Human JEG-3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were blocked with 10% serum for 45 minutes at 24°C. Samples were incubated with primary antibody (1/50) for 1 hour at 24°C. ab6785 (1/100) was used as the secondary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Guo W  et al. Decreased Human Leukocyte Antigen-G Expression by miR-133a Contributes to Impairment of Proinvasion and Proangiogenesis Functions of Decidual NK Cells. Front Immunol 8:741 (2017). Read more (PubMed: 28702027) »
    • James JL  et al. Isolation and characterisation of a novel trophoblast side-population from first trimester placentae. Reproduction 150:449-62 (2015). Read more (PubMed: 26248480) »

    See all 13 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us.

    The antibody is conjugated with neither Fluorescence dye. If this would be the case we would have mentioned this within the name eg. ab7758 Anti-HLA G antibody [MEM-G/9] (PE) and further in the short descriptio...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jeg-3 cell line)
    Loading amount
    100 µg
    Specification
    Jeg-3 cell line
    Gel Running Conditions
    Reduced Denaturing (10)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 20 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (JEG-3)
    Specification
    JEG-3
    Fixative
    NONE
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 21 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (JEG-3)
    Specification
    JEG-3
    Fixation
    NONE
    Permeabilization
    No
    Gating Strategy
    isotype control
    Username

    Abcam user community

    Verified customer

    Submitted Apr 20 2010

    Unfortunately, no information on the reactivity with G*01012 or G*01013 alleles is available.

    Probably not as the cross reactivity is restricted to primates.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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