Overview

  • Product name

    Anti-HLA G antibody [MEM-G/9] (Allophycocyanin)
    See all HLA G primary antibodies
  • Description

    Mouse monoclonal [MEM-G/9] to HLA G (Allophycocyanin)
  • Host species

    Mouse
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity

    This antibody is specific for the native form of HLA G and the soluble HLA G5 isoform in its beta2 microglobulin associated form. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length Human HLA G protein refolded with beta2 microglobulin and peptide.

  • General notes

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.097% Sodium azide
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The conjugate is purified by size exclusion chromatography then adjusted for direct use.
  • Clonality

    Monoclonal
  • Clone number

    MEM-G/9
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.
  • Tissue specificity

    Expressed in trophoblasts.
  • Sequence similarities

    Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B2 microglobulin antibody
    • DADB-15K14.8 antibody
    • HLA 6.0 antibody
    • HLA class I histocompatibility antigen alpha chain G antibody
    • HLA class I histocompatibility antigen, alpha chain G antibody
    • HLA class I molecule antibody
    • HLA G antibody
    • HLA G antigen antibody
    • HLA G histocompatibility antigen class I G antibody
    • HLA G3 antibody
    • HLA-G antibody
    • HLA-G histocompatibility antigen, class I antibody
    • HLA60 antibody
    • HLAG antibody
    • HLAG_HUMAN antibody
    • Major histocompatibility complex class I G antibody
    • MHC class I antigen antibody
    • MHC class I antigen G antibody
    • MHC G antibody
    • T-cell A locus antibody
    • TCA antibody
    see all

Images

  • Double surface staining of HLA-G1 transfectants (viable cells gate).

    ab40915 was used at a recommended concentration of 1-5 μg/mL.

References

This product has been referenced in:

  • Zhao L  et al. Reassessment of HLA-G isoform specificity of MEM-G/9 and 4H84 monoclonal antibodies. Tissue Antigens 80:231-8 (2012). Read more (PubMed: 22738368) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

A suitable Isotype Control to use in Flow Cytometry with ab40915 antibody is https://www.abcam.com/Mouse-IgG1-1F8-Allophycocyanin-Isotype-Control-ab91358.html, as it matches the primary antibody’s host species, isotype, and conjugation.

Read More

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