Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
Purification notesab223657 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 40,45 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||Use a concentration of 2.5 µg/ml.|
RelevanceHLAB protein is involved in the presentation of foreign antigens to the immune system. It belongs to the MHC class I family and contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Cellular localizationCell Membrane
- AS antibody
- HLA B protein antibody
- HLA class I histocompatibility antigen, B alpha chain antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human tonsil with ab223657 at 2.5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-HLAB antibody (ab223657) at 0.1 µg/ml + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
The observed 45kDa band corresponds to the glycosylated form.
ab223657 has not yet been referenced specifically in any publications.