Anti-HLC-3 antibody (ab153913)
Key features and details
- Rabbit polyclonal to HLC-3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HLC-3 antibody -
Description
Rabbit polyclonal to HLC-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human HLC-3 aa 471-646.
Database link: Q9BWU0 -
Positive control
- 293T, A431, Jurkat and Raji whole cell lysates; Human breast carcinoma tissue; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 89 kDa.
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IHC-P |
1/100 - 1/1000. Perform antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 89 kDa. |
IHC-P
1/100 - 1/1000. Perform antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) |
ICC/IF
1/100 - 1/1000. |
Target
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 1 FHA domain. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear. Small amounts are found in the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 22950 Human
- Entrez Gene: 20534 Mouse
- Omim: 602655 Human
- SwissProt: Q9BWU0 Human
- Unigene: 306000 Human
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Alternative names
- FLJ10624 antibody
- FLJ41004 antibody
- HLC-3 antibody
see all
Images
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Anti-HLC-3 antibody (ab153913) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HLC-3 with ab153913 at 1/100 dilution.
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Immunofluorescent analysis of methanol-fixed A431 cells labeling HLC-3 with ab153913 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab153913 has not yet been referenced specifically in any publications.