Key features and details
- Rabbit polyclonal to HLC-3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HLC-3 antibody
DescriptionRabbit polyclonal to HLC-3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human HLC-3 aa 471-646.
Database link: Q9BWU0
- 293T, A431, Jurkat and Raji whole cell lysates; Human breast carcinoma tissue; A431 cells.
Previously labelled as Kanadaptin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab153913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 89 kDa.|
|IHC-P||1/100 - 1/1000. Perform antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)|
|ICC/IF||1/100 - 1/1000.|
Tissue specificityUbiquitously expressed.
Sequence similaritiesContains 1 FHA domain.
Cellular localizationNucleus. Cytoplasm. Mainly nuclear. Small amounts are found in the cytoplasm.
- Information by UniProt
- FLJ10624 antibody
- FLJ41004 antibody
- HLC-3 antibody
Anti-HLC-3 antibody (ab153913) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 89 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling HLC-3 with ab153913 at 1/100 dilution.
Immunofluorescent analysis of methanol-fixed A431 cells labeling HLC-3 with ab153913 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
ab153913 has not yet been referenced specifically in any publications.