Key features and details
- Rabbit polyclonal to hlgB
- Suitable for: WB, ELISA
- Reacts with: Staphylococcus aureus
- Isotype: IgG
Product nameAnti-hlgB antibody
DescriptionRabbit polyclonal to hlgB
SpecificityDue to the high level of homology among the bi-component cytolysins, ab190338 demonstrates cross-reactivity to rHlg C, rLukF-PV and rLukS-PV . The immunogen contained a His-tag, therefore, proteins containing a His-epitope tag may also cross-react. This antibody is an IgG fraction of serum and therefore may have antibodies to other antigens including Staphylococcal antigens as a result of natural exposure and immune response.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Staphylococcus aureus
Recombinant full length protein (His-tag) corresponding to hlgB aa 1-325. Immunogen species: Staphylococcus aureus
MKMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTLYKTTAT ADSDKFKISQILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFS KLYWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISI SNGLSGGLNGNTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNN GWGPYGRDSFHPTYGNELFLAGRQSSAYAGQNFIAQHQMPLLSRSNFNPE FLSVLSHRQDGAKKSKITVTYQREMDLYQIRWNGFYWAGANYKNFKTRTF KSTYEIDWENHKVKLLDTKETENNK
Database link: P0A075
- Recombinant hlg B protein, culture supernatants of Staphylococcus aureus strains USA300 , Newman and NCTC 8325-4.
av190338 can be used for detection of gamma-hemolysin secreted by S. aureus.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab190338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 37 kDa.|
|ELISA||Use at an assay dependent concentration.|
RelevanceToxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
- Gamma hemolysin component B antibody
- H gamma 1 antibody
- H gamma I antibody
All lanes : Anti-hlgB antibody (ab190338) at 0.5 µg/ml
Lane 1 : Culture supernatant of Staphylococcus aureus strain USA300
Lane 2 : Culture supernatant of Staphylococcus aureus strain Newman
Lane 3 : Culture supernatant of Staphylococcus aureus strain NCTC 8325-4
Lane 4 : Culture supernatant of Staphylococcus aureus strain exoprotein negative NCTC 8325-4
Lysates/proteins at 15 µl per lane.
All lanes : Anti-rabbit IgG-AP conjugate
Predicted band size: 37 kDa
Observed band size: <40 kDa why is the actual band size different from the predicted?
Anti-hlgB antibody (ab190338) at 0.5 µg/ml + Recombinant hlg B protein at 0.1 µg
Anti-rabbit IgG-AP conjugate
Predicted band size: 37 kDa
ab190338 has not yet been referenced specifically in any publications.