Product nameAnti-HLTF antibody
See all HLTF primary antibodies
DescriptionRabbit polyclonal to HLTF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to a region within amino acids 196-472 of Human HLTF (UniProt ID: Q14527).
- A549 and HeLa whole cell lysate (ab150035); HeLa cells; Human MB157 xenograft tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab155031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 113 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)|
|ICC/IF||1/100 - 1/1000.|
FunctionHas both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
- Information by UniProt
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
Anti-HLTF antibody (ab155031) at 1/5000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 113 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling HLTF using ab155031 at 1/500 dilution. Lower image costained with Hoechst 33342.
Immunohistochemical analysis of paraffin embedded Human MB157 xenograft tissue, labeling HLTF using ab155031 at 1/500 dilution.
This product has been referenced in:
- Waraky A et al. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem 292:18227-18239 (2017). Read more (PubMed: 28924044) »