Overview

  • Product name

  • Description

    Rabbit polyclonal to HLTF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 196-472 of Human HLTF (UniProt ID: Q14527).

  • Positive control

    • A549 and HeLa whole cell lysate (ab150035); HeLa cells; Human MB157 xenograft tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab155031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
    • Helicase like transcription factor antibody
    • Helicase-like transcription factor antibody
    • HIP116 antibody
    • HIP116A antibody
    • HLTF 1 antibody
    • Hltf antibody
    • HLTF_HUMAN antibody
    • HLTF1 antibody
    • p113 antibody
    • RING finger protein 80 antibody
    • RNF80 antibody
    • SMARC A3 antibody
    • SMARCA 3 antibody
    • SMARCA3 antibody
    • SNF2-like 3 antibody
    • SNF2L3 antibody
    • Sucrose nonfermenting protein 2 like 3 antibody
    • Sucrose nonfermenting protein 2-like 3 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
    • ZBU 1 antibody
    • ZBU1 antibody
    see all

Images

  • Anti-HLTF antibody (ab155031) at 1/5000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 113 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling HLTF using ab155031 at 1/500 dilution. Lower image costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human MB157 xenograft tissue, labeling HLTF using ab155031 at 1/500 dilution.

References

This product has been referenced in:

  • Waraky A  et al. Nuclear insulin-like growth factor 1 receptor phosphorylates proliferating cell nuclear antigen and rescues stalled replication forks after DNA damage. J Biol Chem 292:18227-18239 (2017). Read more (PubMed: 28924044) »
See 1 Publication for this product

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