• Product name
  • Description
    Rabbit polyclonal to HLTF
  • Host species
  • Tested applications
    Suitable for: ChIP, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to HLTF aa 600-700.
    Database link: 6596



Our Abpromise guarantee covers the use of ab17984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB 1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).
IP Use a concentration of 5 µg/ml.


  • Function
    Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
  • Tissue specificity
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
    • Helicase like transcription factor antibody
    • Helicase-like transcription factor antibody
    • HIP116 antibody
    • HIP116A antibody
    • HLTF 1 antibody
    • Hltf antibody
    • HLTF_HUMAN antibody
    • HLTF1 antibody
    • p113 antibody
    • RING finger protein 80 antibody
    • RNF80 antibody
    • SMARC A3 antibody
    • SMARCA 3 antibody
    • SMARCA3 antibody
    • SNF2-like 3 antibody
    • SNF2L3 antibody
    • Sucrose nonfermenting protein 2 like 3 antibody
    • Sucrose nonfermenting protein 2-like 3 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
    • ZBU 1 antibody
    • ZBU1 antibody
    see all


  • SMARCA3 was immunoprecipitated using 0.5mg HeLa whole cell extract, 5µg of Rabbit polyclonal to HLTF and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, HeLa whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab17984.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 113kDa; SMARCA3, non specific bands - 50kDa: We are unsure as to the identity of this extra band.

  • Detection of SMARCA3 by western blot and immunoprecipitation.
    Lane 1:  20 µg of HeLa nuclear extract
    Lane 2:  50 µg of HeLa nuclear extract
    LAne 3:  1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 µg/mg of lysate.
    Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
    Detection:  chemiluminescence with an exposure time of 4 minutes.





    Detection of SMARCA3 by western blot and immunoprecipitation.
    Lane 1:  20 µg of HeLa nuclear extract
    Lane 2:  50 µg of HeLa nuclear extract
    LAne 3:  1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 µg/mg of lysate.
    Anti-SMARCA3 (ab179

  • ab17984 in ChIP of rabbit endometrium nuclear cell lysate. In vivo binding of SMARCA3 (RUSH) to DNA and Egr-1/c-Rel in the rabbit RUSH promoter was confirmed by PCR amplification of products from ChIP assays.

    Lane 1 FX174/HaeIII size markers.

    Lane 2 Single 166-bp amplicons of the SMARCA3 (RUSH) site resulting from ChIP with antibodies to SMARCA3.

    Lanes 3 Negative ChIP control (IgG)

    Lane 4 PCR control (water)

    Lane 5 Single 153-bp amplicons of the Sp1(A) site resulting from ChIP with antibodies to SMARCA3. The SMARCA3 (RUSH) site was identified through antibody-SMARCA3-DNA binding. The Sp1(A) site is an Egr-1/Sp1/3/MAZ/MZF1/c-Rel composite binding site that was identified through antibody-SMARCA3-Egr-1/c-Rel-DNA binding.

    Cross-linking agent: disuccinimydyl glutarate and formaldehyde (0.75%)

    See Abreview


This product has been referenced in:
  • Helmer RA  et al. Helicase-like transcription factor (hltf) regulates g2/m transition, wt1/gata4/hif-1a cardiac transcription networks, and collagen biogenesis. PLoS One 8:e80461 (2013). Mouse . Read more (PubMed: 24278285) »
  • Helmer RA  et al. Role of helicase-like transcription factor (hltf) in the G2/m transition and apoptosis in brain. PLoS One 8:e66799 (2013). Read more (PubMed: 23826137) »

See all 4 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry and interest in our products. As our Western blot image indicates on the product datasheet, ab17984 has been tested and characterized for Western blot on HeLa nuclear extracts so this type of lysate can be applied as posit...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 15 2010

Rabbit Cell lysate - nuclear (endometrium)
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Detection step
Semiquantitative PCR
Positive control
PCR amplification of known postive sites.
Negative control
No antibody for the ChIP, which became a negative control in the PCR reaction, plus a water blank for the PCR reaction.

Prof. Beverly Chilton

Verified customer

Submitted Aug 16 2006


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