Anti-HLTF antibody (ab17984)
Key features and details
- Rabbit polyclonal to HLTF
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HLTF antibody
See all HLTF primary antibodies -
Description
Rabbit polyclonal to HLTF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
Immunogen
Synthetic peptide corresponding to HLTF aa 600-700.
Database link: 6596 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa).
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Notes |
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WB
1/3000 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 114 kDa). |
Target
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Function
Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone. - Information by UniProt
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Database links
- Entrez Gene: 6596 Human
- Entrez Gene: 20585 Mouse
- Entrez Gene: 295568 Rat
- Omim: 603257 Human
- SwissProt: Q14527 Human
- SwissProt: Q6PCN7 Mouse
- Unigene: 3068 Human
- Unigene: 209650 Mouse
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Alternative names
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
see all
Images
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All lanes : Anti-HLTF antibody (ab17984) at 0.5 µg/ml
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : TCMK-1 whole cell lysate
Lane 3 : 4T1 whole cell lysate
Lane 4 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 114 kDa -
Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20 g of HeLa nuclear extractµ
Lane 2: 50µ g of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4 g/mg of lysate.µ
Anti-SMARCA3 (ab17984) was used for western blot analysis at 1/5,000.
Detection: chemiluminescence with an exposure time of 4 minutes.Detection of SMARCA3 by western blot and immunoprecipitation.
Lane 1: 20µg of HeLa nuclear extract
Lane 2: 50µg of HeLa nuclear extract
LAne 3: 1 mg of HeLa nuclear extract immunoprecipitated by anti-SMARCA3 (ab17984), used at 4µg/mg of lysate.
Anti-SMARCA3 (ab179
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab17984 has been referenced in 13 publications.
- Matkovic R et al. TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. Nat Commun 13:66 (2022). PubMed: 35013187
- Kanao R et al. RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance. Life Sci Alliance 5:N/A (2022). PubMed: 35905994
- Helmer RA et al. Helicase-like transcription factor-deletion from the tumor microenvironment in a cell line-derived xenograft model of colorectal cancer reprogrammed the human transcriptome-S-nitroso-proteome to promote inflammation and redirect metastasis. PLoS One 16:e0251132 (2021). PubMed: 34010296
- Kim JJ et al. PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells. Mol Cell 80:327-344.e8 (2020). PubMed: 32966758
- Ostrovsky O et al. Identification of strong intron enhancer in the heparanase gene: effect of functional rs4693608 variant on HPSE enhancer activity in hematological and solid malignancies. Oncogenesis 7:51 (2018). PubMed: 29955035