Anti-HLTF antibody (ab3852)

Submit a review Q&A (2)

Overview

  • Product name
    Anti-HLTF antibody
    See all HLTF primary antibodies
  • Description
    Rabbit polyclonal to HLTF
  • Host species
    Rabbit
  • Specificity
    Recognizes a region between residues 900 and 950 of human HTLF using the numbering for NCBI accession NP_620636.1 (GeneID 6596).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide (Human) - which represents a portion of human HLTF encoded in part by exons 23 and 24.

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab3852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 110 kDa).

Target

  • Function
    Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
  • Tissue specificity
    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization
    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
    • Helicase like transcription factor antibody
    • Helicase-like transcription factor antibody
    • HIP116 antibody
    • HIP116A antibody
    • HLTF 1 antibody
    • Hltf antibody
    • HLTF_HUMAN antibody
    • HLTF1 antibody
    • p113 antibody
    • RING finger protein 80 antibody
    • RNF80 antibody
    • SMARC A3 antibody
    • SMARCA 3 antibody
    • SMARCA3 antibody
    • SNF2-like 3 antibody
    • SNF2L3 antibody
    • Sucrose nonfermenting protein 2 like 3 antibody
    • Sucrose nonfermenting protein 2-like 3 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
    • ZBU 1 antibody
    • ZBU1 antibody
    see all

Images

  • Western blot - Anti-HLTF antibody (ab3852)
    Western blot - Anti-HLTF antibody (ab3852)

    Sample: Nuclear Extract (20µg) from HeLa cells

    Antibody: Affinity purified Rabbit anti-SMARCA 3 (ab3852; 0.5µg/ml)

     

     

    Sample: Nuclear Extract (20µg) from HeLa cells

    Antibody: Affinity purified Rabbit anti-SMARCA 3 (ab3852; 0.5µg/ml)

References

ab3852 has not yet been referenced specifically in any publications.

Publishing research using ab3852? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

whether the band on the western blot is definitly the long isoform or whether it could be the short one? what about the band at 150kD

Read More
Answer

Looking through my archives, I realized that I haven't sent this email to you yet. I am so sorry. Have you meanwhile been able to resolve the question with the ab3852?

I have had contacted the laboratory, which confirmed that the epitope recognized by the antibody should be in both the long and short isoform.This antibody was qualifiedwithNuclear Extract from HeLa cells. We do not know the identity of the protein that migrates just slightly more slowly than the 160K molecular weight marker. Unfortunately we do not know which isoform is the predominant isoform.

The laboratory is producing a new batch. Would you like to try a vial of the new batch?

As far as the band you observed at 105 kD, it would be helpful if we could see the image. Ifyou maybe couldcomplete the attached troubleshooting worksheet, and send it to uswith an image,we would have a better understanding of his experiment.

Again, please apologize for this long delay in my answer. Please let me know if you are still interested in investigating the band at 105kD.

Read More

Question

Where is the immunogen sequence located on the protein?

Read More
Answer

Thank you for contacting us.

The antibody recognizes a region between residues 900 and 950 of human HTLF using the numbering for NCBI accession NP_620636.1 (GeneID 6596).

EAGSPTIMLL SLKAGGVGLN LSAASRVFLM DPAWNPAAED QCFDRCHRLG

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up