- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-HLTF antibody
See all HLTF primary antibodies -
DescriptionRabbit polyclonal to HLTF
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Host speciesRabbit
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SpecificityRecognizes a region between residues 900 and 950 of human HTLF using the numbering for NCBI accession NP_620636.1 (GeneID 6596).
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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Immunogen
Synthetic peptide (Human) - which represents a portion of human HLTF encoded in part by exons 23 and 24.
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Positive control
- Nuclear extract from HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 110 kDa). |
Target
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FunctionHas both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
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Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger. -
Cellular localizationCytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
- Information by UniProt
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Database links
- Entrez Gene: 6596 Human
- Entrez Gene: 20585 Mouse
- Entrez Gene: 295568 Rat
- Omim: 603257 Human
- SwissProt: Q14527 Human
- SwissProt: Q6PCN7 Mouse
- Unigene: 3068 Human
- Unigene: 209650 Mouse
see all -
Alternative names
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
see all
Images
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Western blot - Anti-HLTF antibody (ab3852)Sample: Nuclear Extract (20
µ g) from HeLa cellsAntibody: Affinity purified Rabbit anti-SMARCA 3 (ab3852; 0.5
µ g/ml)Sample: Nuclear Extract (20
µg) from HeLa cells Antibody: Affinity purified Rabbit anti-SMARCA 3 (ab3852; 0.5
µg/ml)
Protocols
References
ab3852 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"