Product nameAnti-HLTF antibody [EPR14761]
See all HLTF primary antibodies
DescriptionRabbit monoclonal [EPR14761] to HLTF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HLTF aa 950 to the C-terminus. The exact sequence is proprietary.
Database link: Q14527
- HeLa, K562 and Jurkat cell lysates; Human infiltrating duct carcinoma of breast and Human ovarian carcinoma tissues; Jurkat cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab183042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionHas both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
Tissue specificityExpressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
- Information by UniProt
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HLTF knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 114 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?
Lanes 1 - 3: Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. Ab183042 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution
Lane 1 : HeLa lysate
Lane 2 : K562 lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 114 kDa
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This product has been referenced in:
- Kaur G et al. Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface. PLoS One 13:e0200211 (2018). Read more (PubMed: 29975766) »
- Masuda Y et al. Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway. Nucleic Acids Res 46:11340-11356 (2018). Read more (PubMed: 30335157) »