Overview

  • Product name

    Anti-HLTF antibody [EPR14761]
    See all HLTF primary antibodies
  • Description

    Rabbit monoclonal [EPR14761] to HLTF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HLTF aa 950 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14527

  • Positive control

    • HeLa, K562 and Jurkat cell lysates; Human infiltrating duct carcinoma of breast and Human ovarian carcinoma tissues; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab183042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

Target

  • Function

    Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
  • Tissue specificity

    Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
    • Helicase like transcription factor antibody
    • Helicase-like transcription factor antibody
    • HIP116 antibody
    • HIP116A antibody
    • HLTF 1 antibody
    • Hltf antibody
    • HLTF_HUMAN antibody
    • HLTF1 antibody
    • p113 antibody
    • RING finger protein 80 antibody
    • RNF80 antibody
    • SMARC A3 antibody
    • SMARCA 3 antibody
    • SMARCA3 antibody
    • SNF2-like 3 antibody
    • SNF2L3 antibody
    • Sucrose nonfermenting protein 2 like 3 antibody
    • Sucrose nonfermenting protein 2-like 3 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a3 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3 antibody
    • ZBU 1 antibody
    • ZBU1 antibody
    see all

Images

  • All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : HLTF knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 114 kDa
    Observed band size: 113 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. Ab183042 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : K562 lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 114 kDa

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

References

This product has been referenced in:

  • Kaur G  et al. Alternative splicing of helicase-like transcription factor (Hltf): Intron retention-dependent activation of immune tolerance at the feto-maternal interface. PLoS One 13:e0200211 (2018). Read more (PubMed: 29975766) »
  • Masuda Y  et al. Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway. Nucleic Acids Res 46:11340-11356 (2018). Read more (PubMed: 30335157) »
See all 2 Publications for this product

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