Product nameAnti-HLX1 antibody
See all HLX1 primary antibodies
DescriptionRabbit polyclonal to HLX1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between residue 375 and 425 of Human HLX1 (NP_068777.1).
- 293T and Hela whole cell lysates
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab128199 was affinity purified using an epitope specific to HLX1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab128199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 51 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionTranscription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.
Tissue specificityLow level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain.
Sequence similaritiesBelongs to the H2.0 homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- H2.0 like homeo box 1 antibody
- H2.0 like homeobox 1 antibody
- H2.0 like homeobox 1 Drosophila antibody
All lanes : Anti-HLX1 antibody (ab128199) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes
Detection of HLX1 by Western blot of immunoprecipitate
ab128199, at 6 µg/ml, immunoprecipitating HLX1 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
ab128199 was used at 1 µg/ml for blotting immunoprecipitated HLX1.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab128199 has not yet been referenced specifically in any publications.