Overview

  • Product name

  • Description

    Rabbit polyclonal to HLX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 375 and 425 of Human HLX1 (NP_068777.1).

  • Positive control

    • 293T and Hela whole cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH: 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab128199 was affinity purified using an epitope specific to HLX1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells as well as maintenance of Th1-specific gene expression. Involved in embryogenesis and hematopoiesis.
  • Tissue specificity

    Low level in normal B and T-cells, high level in activated lymphocytes and monocytes. Also found in thymus, tonsil, bone marrow, developing vessels, and fetal brain.
  • Sequence similarities

    Belongs to the H2.0 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • H2.0 like homeo box 1 antibody
    • H2.0 like homeobox 1 antibody
    • H2.0 like homeobox 1 Drosophila antibody
    • H2.0-like homeobox protein antibody
    • HB24 antibody
    • HLX antibody
    • HLX_HUMAN antibody
    • Homeobox protein HB24 antibody
    • Homeobox protein HLX1 antibody
    see all

Images

  • All lanes : Anti-HLX1 antibody (ab128199) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 3 minutes
  • Detection of HLX1 by Western blot of immunoprecipitate
    ab128199, at 6 µg/ml, immunoprecipitating HLX1 in 293T whole cell lysate (1 mg for IP, 20% of IP loaded).
    ab128199 was used at 1 µg/ml for blotting immunoprecipitated HLX1.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab128199 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab128199.
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